Observation onin situ hybridization and immunocytochemistry of matrix metalloproteinases in rat pancreas

Abstract

In situ hybridization and immunocytochemical techniques were employed to examine the expression of matrix metalloproteinases-1 (MMP-1) and to identify the pattern of its distribution in rat pancreas. The results indicated that the signal of MMP-1 mRNA and MMP-1 positive immunoreaction were detected in some fiberoblasts around interlobular ducts and exocrine cell in margin acinus of some lobules, but the signal of MMP-1 mRNA and MMP-1 positive immunoreaction could not be detected in most of other acinus and islets of pancreas. It is concluded that the expression of MMP-1 in above cells of rat might play an important role in acinar proliferation and differentiation of rat pancreatic tissues.

Keywords

matrix metalloproteinases-1 / in situ hybridization / pancreas / rat

References

[1] GressT M, Muller-PillaschF, LerchM M, et al.. Balance of expression of genes coding for extracellular matrix proteins and extracellular matrix degrading proteases in chronic pancreatitis. Z Gastroenterol, 1994, 32(4): 221-221

[2] MirallesF, BattelinoT, CzernichowP, et al.. TGF-beta plays a key role in morphogenesis of the pancreatic islets of Langerhans by controlling the activity of the matrix metalloproteinase MMP-2. J Cell Biol, 1998, 143(3): 827-827

[3] WillH, HinzmannB. cDNA sequence and mRNA tissue distribution of a novel human matrixmetalloproteinase with a potential transmembrane segment. Eur J Biochem, 1995, 231(3): 602-602

[4] ItoT, ItoM, ShiozawaJ, et al.. Expression of the MMP-1 in human pancreatic carcinoma: relationship with prognostic factor. Mod Pathol, 1999, 12(7): 669-669

[5] ImamuraT, OhshioG, MiseM, et al.. Expression of membrane-type matrix metalloproteinase-1 in human pancreatic adenocarcinomas. J Cancer Res Clin Oncol, 1998, 124(2): 65-65

[6] NgEK, BarentBL, SmithGS, et al.. Decreased type IV collagenase activity in experimental pancreatic fibrosis. J Surg Res, 2001, 96(1): 6-6

[7] Muller-PillaschF, GressT M, YamaguchiH, et al.. The influence of transforming growth factor beta 1 on the expression of genes coding for matrix metalloproteinases and tissue inhibitors of metalloproteinases during regeneration from cerulein-induced pancreatitis. Pancreas, 1997, 15(2): 168-168

[8] MiettinenP J, HuotariM, KoivistoT, et al.. Impaired migration and delayed differentiation of pancreatic islet cells in mice lacking EGF-receptors. Development, 2000, 127(12): 2617-2617