Reversion mutation in dark variants of luminous bacteria and its application in gene toxicant monitoring

Abstract

The luminous intensity of dark variant (S1) separated from photobacterium phosphoreum (A2) was 1/10 000 less than that of wild-type. Ethidium bromide (EB) (0.6 mg/L), Mytomycin C (MC, 0.05 mg/L), 2-amino fluorene (2-AF, 1.0 mg/L) all could strongly induce reversion mutation for S1 within 24 h and increase reversion ratio significantly. The results of experiments indicated that these revertants had stable genetic characteristic and the mutation may take place at gene levels. The mutagenesis to S1 caused by EB, MC and 2-AF was detected and it may be used as a new rapid, simple and sensitive method for gene toxicant monitoring.

Keywords

ethidium bromide / mytomycin C / 2-amino fluorine / dark variant / reversion mutation / gene toxicant monitoring

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