Association of HLA-DRB1, DQB1 alleles with chronic urticaria

Abstract

In order to investigate the association of genotypes of HLA-DRB1 and HLA-DQB1 alleles with the genetic susceptibility of chronic urticaria (CU), genotypes of HLA-DRB1 and HLA-DQB1 genes were detected by polymerase chain reactions with sequence-specific primers (PCR-SSP) in 42 patients with CU (19 men and 23 women, mean age 30.67±12.45 y old as well as 193 racially matched healthy persons in ethnic Han from Hubei provinece. Gene frequencies of HLA-DRB1* 12. * 0901 (RR=3.11, χ²=7.579,P=0.006; RR=2.47, χ²=5.684,P=0.017) were significantly increased in CU patients as compared with that in healthy people. Gene frequencies of HLA-DQB1 * 05 (RR=0.26, χ²=6.683,P=0.01) were significantly decreased in CU patients. It was suggested that CU was found strongly associated with HLA-DRB1 * 12, * 0901 and HLA-DQB1 * 05, the former might be the genetic markers for susceptibility to CU, but the latter might play a resistive role.

Keywords

urticaria, chronic / genes, MHC class II / polymerase chain reaction-sequence specific primer

References

[1] HideM, FrancisD M, GrattanC E H, et al.. Autoantibodies against the high affinity IgE receptor as a cause for histamine release in chronic urticaria. New Engl J Med, 1993, 328: 1599-1599

[2] AseroR. Chronic urticaria: a family study. Ann Allergy Asthma Immunol, 2002, 89: 195-195

[3] BunceM, O’NeillC M, BarnardoM C M N, et al.. Phototyping: comprehensive DNA typing for HLA-A, B, C, DRB1, DRB3, DRB4, DRB5 & DQB1 by PCR with 144 primer mixes utilising sequence-specific primers (PCR-SSP). Tissue Antigens, 1995, 46: 355-355

[4] OlerupO, ZetterquistH. HLA-DR typing by PCR amplification with sequence-specific primers (PCR-SSP) in 2 hours: An alternative t6 serological DR typing in clinical practice including donor-recipient matching in cadaveric transplantations. Tissue Antigens, 1992, 39: 225-225

[5] LeznoffA, SussmanG L. Syndrome of idiopathic chronic urticaria and angioedema with thyroid autoimmunity—a study of 90 patients. J Allergy Clin Immunol, 1989, 84: 66-66

[6] BlumenthalM N, DalmassoA P, RoitmanB, et al.. Lack of linkage between hereditary angioedema and the A and B loci of the HLA system. Vox Sang, 1978, 35: 132-132

[7] EggertJ, ZachariaeH, SvejgaardE, et al.. Hereditary angioneurotic edema and HLA types in two Danish families. Arch Dermatol Res, 1982, 273: 347-347

[8] OlafssonJ H, RoupeG, RydbergL. HLA and lymphocyte histamine sensitivity in mastocytosis. Int Arch Allergy Appl Immunol, 1985, 77: 430-430

[9] LongleyS, PanushR S. Pamilial exercise-induced anaphylaxis. Ann Allergy, 1987, 58: 257-257

[10] Salazar VillaR M, Acosta OrtizR, Mejia OrtegaJ, et al.. Symptomatic dermatographism and HLA antigens. Rev Alerg, 1992, 39: 89-89

[11] O’DonnellB F, O’NeillC M O, FrancisD M, et al.. Human leukocyte antigen Class II associations in chronic idiopathic urticaria. Br J Dermatol, 1999, 140: 853-853

[12] OztasP, OnderM, GonenS, et al.. Is there any relationship between human leucocyte antigen class II and chronic urticaria?. Yonsei Med J, 2004, 45: 392-392

[13] CavanD A, PennyM A, JacobsK H, et al.. The HLA association with Graves disease is sex-specific in Hong Kong Chinese subjects. Clin Endocrinol, 1994, 40: 63-63

[14] GaryW K, WongS H, ChengJ S, et al.. The HLA-DQ associations with Graves disease in Chinese children. Clin Endocrinol, 1999, 50: 493-493

[15] YanagawaT, MangklabruksA, ChangY B, et al.. Human Histocompatibility leukocyte antigen-DQA1 * 0501 allele associated with genetic susceptibility to Graves’ disease in a Caucasian population. J Clin Endocrinol Metab, 1993, 76: 1569-1569

[16] HowellW M, StandringP, WarnerJ A, et al.. HLA class II genotype, HLA-DRB cell surface expression and allergen specific IgE production in atopic and non-atopic members of asthmatic family pedigrees. Clin Exp Allergy, 1999, 29(Suppl 4): 35-35

[17] AbbasA K, LichtmanA H, PoberJ S. Cellular and Molecular Immunology, 1997, Philadelphia, W. B. Saunders Company: 341-61