Effects of 17β-estradiol on the expression of IL-6, IL-11 and NF-κB in human MG-63 osteoblast-like cell line
Wang Yunlin , Liao Eyuan , Xiang Guangda , Dai Ruchun , Xiao Xinhua , Luo Xianghang
Current Medical Science ›› 2006, Vol. 26 ›› Issue (16) : 53 -58.
Effects of 17β-estradiol on the expression of IL-6, IL-11 and NF-κB in human MG-63 osteoblast-like cell line
In order to characterize the effects of 17β estradiol (17β-E2) on the expression of IL-6, IL 11 and NF-κB in the human MG 63 osteoblast like cell line, the expression of IL-6 was detected by RT-PCR. Northern blot and Western blot. The expression of IL-11 was determined by RT-PCR, and NF-κB by Western blot. The results showed that 17β-E2 down-regulated the expression of IL 6 mRNA and protein. IL 11 mRNA and NF κB protein in MG-63 cells. It was suggested that the expression of NF-κB, IL-6 and IL-11 in MG 63 cells could be suppressed by 17β-E2, and this might lend support to estrogen replacement therapy in postmenopausal women.
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