In vivo intratumoral heterogeneity in a dish: scalable forebrain organoid models of embryonal brain tumors for high-throughput personalized drug discovery

Nicole C. Riedel; Carolin Walter; Flavia W. de Faria; Lea Altendorf; Paula Aust; Carolin Göbel; Archana Verma; Annika Ballast; Ivan Bedzhov; Rajanya Roy; Daniel Münter; Erik Schüftan; Thomas K. Albert; Claudia Rössig; Pascal Johann; Barbara von Zezschwitz; Sarah Sandmann; Julian Varghese; Christian Thomas; Ulrich Schüller; Jan M. Bruder; Kornelius Kerl

doi:10.1002/cac2.70074

Cancer Communications ›› 2025, Vol. 45 ›› Issue (12) :1670 -1675. DOI: 10.1002/cac2.70074

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In vivo intratumoral heterogeneity in a dish: scalable forebrain organoid models of embryonal brain tumors for high-throughput personalized drug discovery

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¹. Department of Pediatric Hematology and Oncology, University Children's Hospital Münster, Münster, Germany

². Institute of Medical Informatics, University of Münster, Münster, Germany

³. Department of Pediatric Hematology and Oncology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany

⁴. Research Institute Children's Cancer, University Medical Center Hamburg-Eppendorf, Hamburg, Germany

⁵. Embryonic Self-Organization research group, Max Planck Institute for Molecular Biomedicine, Münster, Germany

⁶. Pediatrics and Adolescent Medicine, Swabian Children's Cancer Center, Experimental pediatrics, University Hospital Augsburg, Augsburg, Germany

⁷. Bavarian Cancer Research Center, Augsburg, Germany

⁸. Department of Pediatric Hematology and Oncology, Charité University Medicine, Berlin, Germany

⁹. Institute of Medical Data Science, Otto-von-Guericke-University Magdeburg, Magdeburg, Germany

¹⁰. Institute of Neuropathology, University Hospital Münster, Münster, Germany

¹¹. 1Institute of Neuropathology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany

¹². Department of Cell and Developmental Biology, Max Planck Institute for Molecular Biomedicine, Münster, Germany

kornelius.kerl@ukmuenster.de

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Nicole C. Riedel, Carolin Walter, Flavia W. de Faria, Lea Altendorf, Paula Aust, Carolin Göbel, Archana Verma, Annika Ballast, Ivan Bedzhov, Rajanya Roy, Daniel Münter, Erik Schüftan, Thomas K. Albert, Claudia Rössig, Pascal Johann, Barbara von Zezschwitz, Sarah Sandmann, Julian Varghese, Christian Thomas, Ulrich Schüller, Jan M. Bruder, Kornelius Kerl. In vivo intratumoral heterogeneity in a dish: scalable forebrain organoid models of embryonal brain tumors for high-throughput personalized drug discovery. Cancer Communications, 2025, 45(12): 1670-1675 DOI:10.1002/cac2.70074

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References

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