Objective: to estimate the possibility of using Danio rerio (Zebrafish) as a test system for assessing the effects of ionizing radiation using an erythrocyte micronuclei test.

Materials and methods: 6 groups of fish (n = 120) were exposed to X-rays in doses of 0, 10, 20, 30, 40, 50 Gy to calculate semi-lethal dose (LD_{50 / 30}). 9 groups of fish (n = 27) were exposed to X-ray at doses of 0 Gy (control), 0.5 Gy, 1.0 Gy, 1.5 Gy, 2.0 Gy, 3.0 Gy, 4.0 Gy, 6,0 Gy, 8.0 Gy for constructing a dose-dependent curve of the occurrence of micronuclei in peripheral blood red blood cells.

Results: the semi-lethal dose (LD_{50 / 30}) for Danio rerio was calculated (24.9 Gy). The frequencies of micronuclei in the erythrocytes of Danio rerio were dose-dependent. Significant data of the frequency of occurrence of micronuclei were found when fish were irradiated at doses of 4.0 Gy, 6.0 Gy and 8.0 Gy.

Shalaev Sergey Alekseevich (born 1939) - surgeon, doctor of medical sciences (1984), professor (1986), honored doctor of the Russian Federation (1995), colonel of medical service (1980). Head of the department of thoracic (hospital) surgery from 1988 to 1994. Priority research in the field of surgery of lung cancer, purulent-destructive diseases of the lungs and pleura, injuries and injuries of the chest and chest cavity, resuscitation and anesthesia in thoracic surgery and pulmonology (1 figure, bibliography: 8 refs).

The purpose of this review is to systematize scientific data and to assess the prospects for using mesenchymal stem cells (MSCs) to treat injury in the Arctic. Here, we considered possible sources of MSCs, barriers and limitations of their use and possible strategies to improve their therapeutic outcome (bibliography: 33 refs).

Brief information on the genes of the dopamine system, one of the most important proteins involved in the formation of stress and stress resistance, is presented. The data on the synthesis of dopamine, the COMT and MAO genes that take part in its metabolism, the role of dopamine in the activities of the reward system, the association of dopamine and Parkinson's disease, schizophrenia, attention deficit hyperactivity disorder. The gene polymorphism PRTFDC1 (rs6482463 (A; G)), which, as shown, is associated with a predisposition to stress, is described (bibliography: 48 refs).

In recent years, the epidemiological situation of tuberculosis in Russia and the Armed Forces of the Russian Federation remains tense for a number of reasons. Including this fact is caused by untimely, sometimes posthumous, detection of tuberculosis. Late diagnosis of the disease is associated with a low-symptom or asymptomatic clinical course, the absence of pathognomonic radiological, laboratory, immunological signs of the disease, often the absence of bacterial excretion. The detection of tuberculosis is also difficult in the differential diagnosis with sarcoidosis, which is characterized by very similar clinical, radiological and even pathomorphological signs in the form of granulomatous inflammation. An analysis of a clinical case was carried out in which for a young man for about 1.5 years tuberculosis proceeded along the mask of sarcoidosis, which led to the generalization of a specific infection. Even after the start of an intensive examination using modern diagnostic methods, it took two months to go from the diagnoses of “lacunar tonsillitis”, “community-acquired pneumonia”, “sarcoidosis of the respiratory system”, “lymphoproliferative disease of unknown etiology” to the diagnosis “generalized tuberculosis” confirmed histologically. A decisive role in identifying the disease was played by biopsy methods followed by histological verification of the diagnosis. Thus, it is advisable for all patients with suspected sarcoidosis who are admitted to military medical organizations, regardless of the stage of the process and the results of the previous examination, to consult a TB specialist, performing a diagnostic minimum to exclude active tuberculosis (bibliography: 7 refs).

Objective: to estimate the possibility of using Danio rerio (Zebrafish) as a test system for assessing the effects of ionizing radiation using an erythrocyte micronuclei test. Materials and methods: 6 groups of fish (n = 120) were exposed to X-rays in doses of 0, 10, 20, 30, 40, 50 Gy to calculate semi-lethal dose (LD50 / 30). 9 groups of fish (n = 27) were exposed to X-ray at doses of 0 (control), 0.5, 1.0, 1.5, 2.0, 3.0, 4.0, 6,0, 8.0 Gy for constructing a dose-dependent curve of the occurrence of micronuclei in peripheral blood red blood cells. Results: the semi-lethal dose (LD50 / 30) for Danio rerio was calculated (24.9 Gy). The frequencies of micronuclei in the erythrocytes of Danio rerio were dose-dependent. Significant data of the frequency of occurrence of micronuclei were found when fish were irradiated at doses of 4.0, 6.0 and 8.0 Gy. Conclusion: Danio rerio fish are characterized by extreme radioresistance, thereby we suggest the prospect of their use in radiobiology as a lab test system for assessing the effectiveness of factors that induce mutagenic effects of ionizing radiation (1 figure, 1 table, bibliography: 11 refs).

In the article, the author talks about his experience in an innovative process to create a biomedical cell product. From the idea to the production site. This process was started from the moment of choosing a lot of State science competition. Brainstorming was held, ideas were generated, tender documentation was prepared. Subsequently, the project within the framework of the competition was successfully completed. In addition to the ex vivo gene therapy drug developed as part of the competition, additional important results were obtained. So, biomarkers with good diagnostic potential for endometriosis were discovered. The author also addresses the shortcomings of the existing grant system to support innovative projects in the field of biomedicine.

The article is devoted to questions of treatment and diagnostic approach, the evacuation tactics at thermal burns of the Navy personnel. The main characteristics, the criteria of the prognosis of a burn trauma are submitted. The algorithms of antishock actions, therapy of a burn disease and inhalation defeat of airways are described. Also the attention is paid to the problems of inhalation poisoning with ship fire products (mainly, carbon monoxide) and components of volume chemical fire extinguishing system (chladones and derivatives of their thermal decomposition), acute nitrogen-induced hypoxia, acute overheating, which are capable to significantly worsen a clinical status and the forecast at burned seamen. The materials of article were included into the textbook on naval therapy published in Russian Military Medical Academy in 2015 (5 figs, 2 tables, bibliography: 6 refs).

Sergey Alekseevich Shalaev (born 1939) - surgeon, doctor of medical sciences (1984), professor (1986), honored doctor of the Russian Federation (1995), colonel of medical service (1980). Head of the department of thoracic (hospital) surgery from 1988 to 1994. Priority research in the field of surgery of lung cancer, purulent-destructive diseases of the lungs and pleura, injuries and injuries of the chest and chest cavity, resuscitation and anesthesia in thoracic surgery and pulmonology (1 figure, bibliography: 8 refs).

The revolutionary events of 1917-1921 played a huge social significance in the life of the Russian state. They stirred up the masses of the St. Petersburg workers and matrons of Kronstadt. Thanks to the dedicated work of doctors, medical assistants, nurses and nurses of the Kronstadt Marine Hospital, all patients were provided with the necessary medical care during the revolutionary events (7 figs, bibliography: 6 refs).

This paper reviews stress related healthcare workers mental problems that occur in adverse situation of COVID-19 pandemic. We discuss acute stress reactions, acute and posttraumatic stress disorders screening methods, strategy of emergency psychological assistance and prevention of mental health complications (2 figs, bibliography: 16 refs).