DANIO RERIO (ZEBRAFISH) AS A MODEL FOR RADIOBIOLOGICAL RESEARCH

Abstract

Objective: to estimate the possibility of using Danio rerio (Zebrafish) as a test system for assessing the effects of ionizing radiation using an erythrocyte micronuclei test. Materials and methods: 6 groups of fish (n = 120) were exposed to X-rays in doses of 0, 10, 20, 30, 40, 50 Gy to calculate semi-lethal dose (LD50 / 30). 9 groups of fish (n = 27) were exposed to X-ray at doses of 0 (control), 0.5, 1.0, 1.5, 2.0, 3.0, 4.0, 6,0, 8.0 Gy for constructing a dose-dependent curve of the occurrence of micronuclei in peripheral blood red blood cells. Results: the semi-lethal dose (LD50 / 30) for Danio rerio was calculated (24.9 Gy). The frequencies of micronuclei in the erythrocytes of Danio rerio were dose-dependent. Significant data of the frequency of occurrence of micronuclei were found when fish were irradiated at doses of 4.0, 6.0 and 8.0 Gy. Conclusion: Danio rerio fish are characterized by extreme radioresistance, thereby we suggest the prospect of their use in radiobiology as a lab test system for assessing the effectiveness of factors that induce mutagenic effects of ionizing radiation (1 figure, 1 table, bibliography: 11 refs).

Keywords

Danio rerio / Zebrafish / bioindication / Danio rerio / in vivo model / model organism / radiobiological model / Zebrafish

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RIGHTS & PERMISSIONS

Livanova A.A., Esin T.A., Ilyichev I.V., Zavirsky A.V., Rakin A.I., Kravtsov V.Y.