DANIO RERIO (ZEBRAFISH) AS A MODEL FOR RADIOBIOLOGICAL RESEARCH

Abstract

Objective: to estimate the possibility of using Danio rerio (Zebrafish) as a test system for assessing the effects of ionizing radiation using an erythrocyte micronuclei test.

Materials and methods: 6 groups of fish (n = 120) were exposed to X-rays in doses of 0, 10, 20, 30, 40, 50 Gy to calculate semi-lethal dose (LD_{50 / 30}). 9 groups of fish (n = 27) were exposed to X-ray at doses of 0 Gy (control), 0.5 Gy, 1.0 Gy, 1.5 Gy, 2.0 Gy, 3.0 Gy, 4.0 Gy, 6,0 Gy, 8.0 Gy for constructing a dose-dependent curve of the occurrence of micronuclei in peripheral blood red blood cells.

Results: the semi-lethal dose (LD_{50 / 30}) for Danio rerio was calculated (24.9 Gy). The frequencies of micronuclei in the erythrocytes of Danio rerio were dose-dependent. Significant data of the frequency of occurrence of micronuclei were found when fish were irradiated at doses of 4.0 Gy, 6.0 Gy and 8.0 Gy.

Conclusion: Danio rerio fish are characterized by extreme radioresistance, thereby we suggest the prospect of their use in radiobiology as a lab test system for assessing the effectiveness of factors that induce mutagenic effects of ionizing radiation.

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Keywords

Danio rerio / Zebrafish / bioindication / radiobiological model / model organism / in vivo model

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Livanova A.A.