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Frontiers of Materials Science

Front. Mater. Sci.    2016, Vol. 10 Issue (2) : 113-121     DOI: 10.1007/s11706-016-0339-7
The multifunctional wound dressing with core–shell structured fibers prepared by coaxial electrospinning
Qilin WEI1,Feiyang XU1,Xingjian XU1,Xue GENG1,2,Lin YE1,2,*(),Aiying ZHANG1,2,Zengguo FENG1,2
1. School of Materials Science and Engineering, Beijing Institute of Technology, Beijing 100081, China
2. Beijing Key Laboratory of Construction Tailorable Advanced Functional Materials and Green Applications, Beijing 100081, China
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The non-woven wound dressing with core–shell structured fibers was prepared by coaxial electrospinning. The polycaprolactone (PCL) was electrospun as the fiber’s core to provide mechanical strength whereas collagen was fabricated into the shell in order to utilize its good biocompatibility. Simultaneously, the silver nanoparticles (Ag-NPs) as anti-bacterial agent were loaded in the shell whereas the vitamin A palmitate (VA) as healing-promoting drug was encapsulated in the core. Resulting from the fiber’s core–shell structure, the VA released from the core and Ag-NPs present in the shell can endow the dressing both heal-promoting and anti-bacteria ability simultaneously, which can greatly enhance the dressing’s clinical therapeutic effect. The dressing can maintain high swelling ratio of 190% for 3 d indicating its potential application as wet dressing. Furthermore, the dressing’s anti-bacteria ability against Staphylococcus aureus was proved by in vitro anti-bacteria test. The in vitro drug release test showed the sustainable release of VA within 72 h, while the cell attachment showed L929 cells can well attach on the dressing indicating its good biocompatibility. In conclusion, the fabricated nanofibrous dressing possesses multiple functions to benefit wound healing and shows promising potential for clinical application.

Keywords coaxial electrospinning      core–shell structure      multifunctional wound dressing      anti-bacteria      heal-promoting     
Corresponding Authors: Lin YE   
Online First Date: 22 April 2016    Issue Date: 11 May 2016
 Cite this article:   
Qilin WEI,Feiyang XU,Xingjian XU, et al. The multifunctional wound dressing with core–shell structured fibers prepared by coaxial electrospinning[J]. Front. Mater. Sci., 2016, 10(2): 113-121.
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Qilin WEI
Feiyang XU
Xingjian XU
Lin YE
Aiying ZHANG
Zengguo FENG
Fig.1  (a) Schematic figure of core–shell fiber. (b)(c)(d) SEM images of electrospinning fibers under different core–shell feeding ratios (feeding rate (core) : feeding rate (shell) = 3:1 (b), 2:1 (c), and 1:1 (d)).
Fig.2  TEM images of electrospinning fibers under different core?shell feeding ratio: (a) 3:1; (b) 2:1; (c) 1:1.
Fig.3  IR spectra of PCL, collagen, VA and the dressing.
Fig.4  The swelling ratio of the dressings.
Fig.5  The characterization of Ag-NPs in the dressing: (a) UV spectrum of Ag-NPs; (b)(c) XRD patterns of Ag-NPs.
Fig.6  Anti-bacteria evaluation against Staphylococcus aureus: (a) control group; (b) dressings loaded with Ag-NPs and VA.
Fig.7  In vitro release curve of vitamin A palmitate.
Fig.8  SEM images of L929 cell attachment on dressings.
1 Mogoşanu G D, Grumezescu A M. Natural and synthetic polymers for wounds and burns dressing. International Journal of Pharmaceutics, 2014, 463(2): 127–136
2 Boateng J S, Matthews K H, Stevens H N E, . Wound healing dressings and drug delivery systems: a review. Journal of Pharmaceutical Sciences, 2008, 97(8): 2892–2923
3 Winter G D. Formation of the scab and the rate of epithelization of superficial wounds in the skin of the young domestic pig. Nature, 1962, 193(4812): 293–294
4 Zahedi P, Rezaeian I, Ranaei-Siadat S O, . A review on wound dressings with an emphasis on electrospun nanofibrous polymeric bandages. Polymers for Advanced Technologies, 2010, 21: 77–95
5 Jiang H, Hu Y, Zhao P, . Modulation of protein release from biodegradable core‒shell structured fibers prepared by coaxial electrospinning. Journal of Biomedical Materials Research Part B: Applied Biomaterials, 2006, 79B(1): 50–57
6 Shao W, He J, Sang F, . Coaxial electrospun aligned tussah silk fibroin nanostructured fiber scaffolds embedded with hydroxyapatite-tussah silk fibroin nanoparticles for bone tissue engineering. Materials Science and Engineering C, 2016, 58(C): 342–351
7 Agarwal S, Wendorff J H, Greiner A. Use of electrospinning technique for biomedical applications. Polymer, 2008, 49(26): 5603–5621
8 Liang D, Hsiao B S, Chu B. Functional electrospun nanofibrous scaffolds for biomedical applications. Advanced Drug Delivery Reviews, 2007, 59(14): 1392–1412
9 Wu H, Hu L, Rowell M W, . Electrospun metal nanofiber webs as high-performance transparent electrode. Nano Letters, 2010, 10(10): 4242–4248
10 Sridhar R, Lakshminarayanan R, Madhaiyan K, . Electrosprayed nanoparticles and electrospun nanofibers based on natural materials: applications in tissue regeneration, drug delivery and pharmaceuticals. Chemical Society Reviews, 2015, 44(3): 790–814
11 Wang R, Liu Y, Li B, . Electrospunnanofibrous membranes for high flux microfiltration. Journal of Membrane Science, 2012, 392‒393: 167–174
12 Martinez-Sanz M, Lopez-Rubio A, Villano M, . Production of bacterial nanobiocomposites of polyhydroxyalkanoates derived from waste and bacterial nanocellulose by the electrospinning enabling melt compounding method. Journal of Applied Polymer Science, 2016, 133(2): 42486
13 Li Z, Zhang H, Zheng W, . Highly sensitive and stable humidity nanosensors based on LiCl doped TiO2 electrospun nanofibers. Journal of the American Chemical Society, 2008, 130(15): 5036–5037
14 Holmes B, Fang X, Zarate A, . Enhanced human bone marrow mesenchymal stem cell chondrogenic differentiation in electrospun constructs with carbon nanomaterials. Carbon, 2016, 97: 1–13
15 Sun Z, Zussman E, Yarin A L, . Compound core‒shell polymer nanofibers by Co-electrospinning. Advanced Materials, 2003, 15(22): 1929–1932
16 Hu X, Liu S, Zhou G, . Electrospinning of polymeric nanofibers for drug delivery applications. Journal of Controlled Release, 2014, 185(C): 12–21
17 Yarin A L. Coaxial electrospinning and emulsion electrospinning of core-shell fibers. Polymers for Advanced Technologies, 2011, 22(3): 310–317
18 Li H, Zhao C, Wang Z, . Controlled release of PDGF-bb by coaxial electrospun dextran/poly(L-lactide-co-ϵ-caprolactone) fibers with an ultrafine core/shell structure. Journal of Biomaterials Science. Polymer Edition, 2010, 21(6‒7): 803–819
19 Jia X, Zhao C, Li P, . Sustained release of VEGF by coaxial electrospun dextran/PLGA fibrous membranes in vascular tissue engineering. Journal of Biomaterials Science. Polymer Edition, 2011, 22(13): 1811–1827
20 Rho K S, Jeong L, Lee G, . Electrospinning of collagen nanofibers: effects on the behavior of normal human keratinocytes and early-stage wound healing. Biomaterials, 2006, 27(8): 1452–1461
21 Chattopadhyay S, Raines R T. Review collagen-based biomaterials for wound healing. Biopolymers, 2014, 101(8): 821–833
22 Han J, Lazarovici P, Pomerantz C, . Co-electrospun blends of PLGA, gelatin, and elastin as potential nonthrombogenic scaffolds for vascular tissue engineering. Biomacromolecules, 2011, 12(2): 399–408
23 Zeugolis D I, Khew S T, Yew E S Y, . Electro-spinning of pure collagen nano-fibres - just an expensive way to make gelatin? Biomaterials, 2008, 29(15): 2293–2305
24 Abdelgawad A M, Hudson S M, Rojas O J. Antimicrobial wound dressing nanofiber mats from multicomponent (chitosan/silver-NPs/polyvinyl alcohol) systems. Carbohydrate Polymers, 2014, 100: 166–178
25 Wu J, Zheng Y, Song W, . In situ synthesis of silver-nanoparticles/bacterial cellulose composites for slow-released antimicrobial wound dressing. Carbohydrate Polymers, 2014, 102(4): 762–771
26 Martins N C T, Freire C S R, Pinto R J B, . Electrostatic assembly of Ag nanoparticles onto nanofibrillated cellulose for antibacteria paper products. Cellulose, 2012, 19(4): 1425–1436
27 Hartong D T, Berson E L, Dryja T P. Retinitis pigmentosa. Lancet, 2006, 368(9549): 1795–1809
28 Leonardi G R, Campos P M B G M. Influence of glycolic acid as a component of different formulations on skin penetration by vitamin A palmitate. Journal of Cosmetic Science, 1998, 49(1): 23–32
29 Sun B, Duan B, Yuan X. Preparation of core/shell PVP/PLA ultrafine fibers by coaxial electrospinning. Journal of Applied Polymer Science, 2006, 102(1): 39–45
30 Liao I C, Chew S Y, Leong K W. Aligned core‒shell nanofibers delivering bioactive proteins. Nanomedicine, 2006, 1(4): 465–471
31 Catledge S A, Clem W C, Shrikishen N, . An electrospun triphasic nanofibrous scaffold for bone tissue engineering. Biomedical Materials, 2007, 2(2): 142–150
32 Chang M C, Tanaka J. FT-IR study for hydroxyapatite/collagen nanocomposite cross-linked by glutaraldehyde. Biomaterials, 2002, 23(24): 4811–4818
33 Pezeshki A, Ghanbarzadeh B, Mohammadi M, . Encapsulation of vitamin A palmitate in nanostructured lipid carrier (NLC)-effect of surfactant concentration on the formulation properties. Advanced Pharmaceutical Bulletin, 2014, 4(6): 563–568
35 Choi J S, Lee S J, Christ G J, . The influence of electrospun aligned poly(ϵ-caprolactone)/collagen nanofiber meshes on the formation of self-aligned skeletal muscle myotubes. Biomaterials, 2008, 29(19): 2899–2906
36 Bedran-Russo A K B, Pereira P N R, Duarte W R, . Application of crosslinkers to dentin collagen enhances the ultimate tensile strength. Journal of Biomedical Materials Research Part B: Applied Biomaterials, 2007, 80B(1): 268–272
37 Gümüşderelioğlu M, Dalkıranoğlu S, Aydın R S T, . A novel dermal substitute based on biofunctionalized electrospun PCL nanofibrous matrix. Journal of Biomedical Materials Research Part A, 2011, 98A(3): 461–472
38 Nguyen T H, Kim Y H, Song H Y, . Nano Ag loaded PVA nano-fibrous mats for skin applications. Journal of Biomedical Materials Research Part B: Applied Biomaterials, 2011, 96B(2): 225–233
39 Singh N, Khanna P K. In situ synthesis of silver nano-particles in polymethylmethacrylate. Materials Chemistry and Physics, 2007, 104(2‒3): 367–372
40 Duell E A, Kang S, Voorhees J J. Unoccluded retinol penetrates human skin in vivo more effectively than unoccluded retinyl palmitate or retinoic acid. The Journal of Investigative Dermatology, 1997, 109(3): 301–305
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