Multiple forms of vitamin B₆ regulate salt tolerance by balancing ROS and abscisic acid levels in maize root

Abstract

Salt stress causes osmotic stress, ion toxicity and oxidative stress, inducing the accumulation of abscisic acid (ABA) and excessive reactive oxygen species (ROS) production, which further damage cell structure and inhibit the development of roots in plants. Previous study showed that vitamin B₆ (VB₆) plays a role in plant responses to salt stress, however, the regulatory relationship between ROS, VB₆ and ABA under salt stress remains unclear yet in plants. In our study, we found that salt stress-induced ABA accumulation requires ROS production, in addition, salt stress also promoted VB₆ (including pyridoxamine (PM), pyridoxal (PL), pyridoxine (PN), and pyridoxal 5′-phosphate (PLP)) accumulation, which involved in ROS scavenging and ABA biosynthesis. Furthermore, VB₆-deficient maize mutant small kernel2 (smk2) heterozygous is more susceptible to salt stress, and which failed to scavenge excessive ROS effectively or induce ABA accumulation in maize root under salt stress, interestingly, which can be restored by exogenous PN and PLP, respectively. According to these results, we proposed that PN and PLP play an essential role in balancing ROS and ABA levels under salt stress, respectively, it laid a foundation for VB₆ to be better applied in crop salt resistance than ABA.

Keywords

Vitamin B₆ / Abscisic acid / Reactive oxygen species / Salt stress / smk2

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Funding

National Natural Science Foundation of China(32072500, 31801867, 31872925); Youth Innovation Technology Project of Higher School in Shandong Province(2019KJE011); Shandong Provincial Key Research and Development Plan((2021TZXD007, 2019GNC106152, 2020CXGC010803); Northwest A & F University;