A secreted catalase contributes to Puccinia striiformis resistance to host-derived oxidative stress

Abstract

Plants can produce reactive oxygen species (ROS) to counteract pathogen invasion, and pathogens have also evolved corresponding ROS scavenging strategies to promote infection and pathogenicity. Catalases (CATs) have been found to play pivotal roles in detoxifying H₂O₂ formed by superoxide anion catalyzed by superoxide dismutases (SODs). However, few studies have addressed H₂O₂ removing during rust fungi infection of wheat. In this study, we cloned a CAT gene PsCAT1 from Puccinia striiformis f. sp. tritici (Pst), which encodes a monofunctional heme-containing catalase. PsCAT1 exhibited a high degree of tolerance to pH and temperature, and forms high homopolymers.

Heterologous complementation assays in Saccharomyces cerevisiae reveal that the signal peptide of PsCAT1 is functional. Overexpression of PsCAT1 enhanced S. cerevisiae resistance to H₂O₂. Transient expression of PsCAT1 in Nicotiana benthamiana suppressed Bax-induced cell death. Knockdown of PsCAT1 using a host-induced gene silencing (HIGS) system led to the reduced virulence of Pst, which was correlated to H₂O₂ accumulation in HIGS plants. These results indicate that PsCAT1 acts as an important pathogenicity factor that facilitates Pst infection by scavenging host-derived H₂O₂.

Keywords

Wheat stripe rust / Puccinia striiformis f. sp. tritici / Catalase / Reactive oxygen species / Host-induced gene silencing

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Funding

National Natural Science Foundation of China(U2003118); Natural Science Basic Research Plan in Shaanxi Province of China(2020JZ-12); National “111 plan”(BP0719026); Shaanxi Innovation Team Project(2018TD-004)