Integration of a CO2-fixation pathway and PAN2 truncation in Saccharomyces cerevisiae for low-carbon single-cell protein production

Sujeong Park , Sooah Kim , Dong-Shin Kim , Sun-Ki Kim , Jin-Soo Park , Sungmin Hwang , Soo Rin Kim

Systems Microbiology and Biomanufacturing ›› 2026, Vol. 6 ›› Issue (3) : 83

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Systems Microbiology and Biomanufacturing ›› 2026, Vol. 6 ›› Issue (3) :83 DOI: 10.1007/s43393-026-00486-0
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Integration of a CO2-fixation pathway and PAN2 truncation in Saccharomyces cerevisiae for low-carbon single-cell protein production
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Carbon dioxide fixation / Flux balance analysis / PAN2 truncation / Xylose fermentation

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Sujeong Park, Sooah Kim, Dong-Shin Kim, Sun-Ki Kim, Jin-Soo Park, Sungmin Hwang, Soo Rin Kim. Integration of a CO2-fixation pathway and PAN2 truncation in Saccharomyces cerevisiae for low-carbon single-cell protein production. Systems Microbiology and Biomanufacturing, 2026, 6(3): 83 DOI:10.1007/s43393-026-00486-0

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References

[1]

Albers E, Laizé V, Blomberg A, Hohmann S, Gustafsson L. Ser3p (Yer081wp) and Ser33p (Yil074cp) are phosphoglycerate dehydrogenases in Saccharomyces cerevisiae. J Biol Chem, 2003, 278: 10264-10272

[2]

Choi KR, Ahn Y-J, Lee SY. Bacterial conversion of CO2 to organic compounds. J CO2 Util, 2022, 58: 101929

[3]

Do S-H, Lee T-G, Kim S-K. Enhancing protein content in wild-type Saccharomyces cerevisiae via random mutagenesis and optimized fermentation conditions. J Microbiol Biotechnol, 2024, 34: 1912

[4]

Ebrahim A, Lerman JA, Palsson BO, Hyduke DR. COBRApy: constraints-based reconstruction and analysis for Python. BMC Syst Biol, 2013, 7: 74

[5]

Filonchyk M, Peterson MP, Zhang L, Hurynovich V, He Y. Greenhouse gases emissions and global climate change: examining the influence of CO2, CH4, and N2O. Sci Total Environ, 2024, 935: 173359

[6]

Hosaka K, Nikawa J-i, Kodaki T, Yamashita S. A dominant mutation that alters the regulation of INO1 expression in Saccharomyces cerevisiae. J Biochem, 1992, 111: 352-358

[7]

Huang M, Jin Z, Ni H, Zhang P, Li H, Liu J, et al.. Engineering the xylose metabolism of Saccharomyces cerevisiae for ethanol and single cell protein bioconversion. Biomass Bioenergy, 2024, 190: 107372

[8]

Jeong D, Oh EJ, Ko JK, Nam J-O, Park H-S, Jin Y-S, et al.. Metabolic engineering considerations for the heterologous expression of xylose-catabolic pathways in Saccharomyces cerevisiae. PLoS ONE, 2020, 15: e0236294

[9]

Khunnonkwao P, Thitiprasert S, Jaiaue P, Khumrangsee K, Cheirsilp B, Thongchul N. The outlooks and key challenges in renewable biomass feedstock utilization for value-added platform chemical via bioprocesses. Heliyon, 2024, 10: e30830

[10]

Kierans SJ, Taylor CT. Glycolysis: a multifaceted metabolic pathway and signaling hub. J Biol Chem, 2024, 300: 107906

[11]

Kim S, Lee DY, Wohlgemuth G, Park HS, Fiehn O, Kim KH. Evaluation and optimization of metabolome sample preparation methods for Saccharomyces cerevisiae. Anal Chem, 2013, 85: 2169-2176

[12]

Kim SR, Kim S-J, Kim S-K, Seo S-O, Park S, Shin J, et al.. Yeast metabolic engineering for carbon dioxide fixation and its application. Bioresour Technol, 2022, 346: 126349

[13]

Kim S, Jeong D, Jang B, Park S, Oh EJ, Kim IJ, et al.. Coupled engineering strategy of CYB2 deletion and ACS1 overexpression improves cellulosic lactic acid production by Saccharomyces cerevisiae. Biomass Bioenergy, 2024, 185: 107249

[14]

Kuzman M, Ata Ö, Mattanovich D. Advancing yeast metabolism for a sustainable single carbon bioeconomy. FEMS Yeast Res, 2025

[15]

Lee YO, Do SH, Won JY, Chin YW, Chewaka LS, Park BR, et al.. Inverse metabolic engineering for improving protein content in Saccharomyces cerevisiae. Biotechnol J, 2023, 18: 2300014

[16]

Li Y, Wang C, Huang R, Zhang S, Yin J, Zhang J, et al.. Production of single-cell protein from vinegar residue by Rhodotorula glutinis and techno-economic analysis. Bioresour Technol, 2025, 422: 132252

[17]

Mo ML, Palsson , Herrgård MJ. Connecting extracellular metabolomic measurements to intracellular flux states in yeast. BMC Syst Biol, 2009, 3: 37

[18]

Orth JD, Thiele I, Palsson . What is flux balance analysis?. Nat Biotechnol, 2010, 28: 245-248

[19]

Pan Z, Guo Y, Rong W, Wang S, Cui K, Cai W, et al.. Single-cell protein production from CO2 and electricity with a recirculating anaerobic-aerobic bioprocess. Environ Sci Ecotechnol, 2025, 24: 100525

[20]

Park S, Park B-R, Jeong D, Park J, Ko JK, Kim S-J, et al.. Functional expression of RuBisCO reduces CO2 emission during fermentation by engineered Saccharomyces cerevisiae. Process Biochem, 2023, 134: 286-293

[21]

Qin N, Li L, Wan X, Ji X, Chen Y, Li C, et al.. Increased CO2 fixation enables high carbon-yield production of 3-hydroxypropionic acid in yeast. Nat Commun, 2024, 15: 1591

[22]

Sahoo A, Das PK, Veeranki VD, Patra S. Engineered Saccharomyces cerevisiae for sustainable biobased fuel production: overcoming bottlenecks and implementing strategies. Renew Sustain Energy Rev, 2025, 211: 115352

[23]

Tian Y, Li J, Meng J, Li J. High-yield production of single-cell protein from starch processing wastewater using co-cultivation of yeasts. Bioresour Technol, 2023, 370: 128527

[24]

Tudek A, Krawczyk PS, Mroczek S, Tomecki R, Turtola M, Matylla-Kulińska K, et al.. Global view on the metabolism of RNA poly(A) tails in yeast Saccharomyces cerevisiae. Nat Commun, 2021, 12: 4951

[25]

Wolf J, Passmore LA. mRNA deadenylation by Pan2–Pan3. Biochem Soc Trans, 2014, 42: 184-187

[26]

Xia P-F, Zhang G-C, Walker B, Seo S-O, Kwak S, Liu J-J, et al.. Recycling carbon dioxide during xylose fermentation by engineered Saccharomyces cerevisiae. ACS Synth Biol, 2017, 6: 276-283

[27]

Yu ZY, Zhu WB, Gao H, Hu CY, Zhang CQ, Meng YH. Rubisco-centric strategies for carbon conservation in synthetic biology. J Agric Food Chem, 2025, 73: 25691-25708

[28]

Zhang C, Fei Q, Fu R, Lackner M, Zhou YJ, Tan T. Economic and sustainable revolution to facilitate one-carbon biomanufacturing. Nat Commun, 2025, 16: 4896

Funding

National Research Foundation of Korea(RS-2024- 00439872)

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Jiangnan University

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