Enhancing γ-aminobutyric acid production by Enterococcus faecalis through atmospheric and room temperature plasma mutagenesis, adaptive evolution, and systematic fermentation optimization
Rumeng Han , Kun Qi , Shengming Gu , Qing Ding , Yimei Wang , Min Zhang , Mengkai Hu , Zhenglian Xue , Xiangfei Li
Systems Microbiology and Biomanufacturing ›› 2026, Vol. 6 ›› Issue (3) : 68
Enterococcus faecalis / ARTP mutagenesis / Adaptive evolution / Fermentation medium optimization
| [1] |
|
| [2] |
|
| [3] |
|
| [4] |
|
| [5] |
Wang Q, Luo Y, Zhu H, Liu X, Xue M, Yang G, Chen Y, Chen S, Wen Z. In vitro gastrointestinal digestion of grifola frondosa polysaccharides and their enhancement of GABA production via gut microbiota modulation. Nutrients 2025; 3332. |
| [6] |
|
| [7] |
Iorizzo M, Paventi G, Di Martino C. Biosynthesis of gamma-aminobutyric acid (GABA) by Lactiplantibacillus plantarum in fermented food production. Curr Issues Mol Biol 2024; 200–20. |
| [8] |
|
| [9] |
Yogeswara IBA, Maneerat S, Haltrich D. Glutamate decarboxylase from lactic acid bacteria-a key enzyme in GABA synthesis. Microorganisms. 2020; 8(12): 1923. https://doi.org/10.3390/microorganisms8121923 |
| [10] |
|
| [11] |
|
| [12] |
|
| [13] |
Luo J, Huang G, Zheng M, Ou C, Chen Q, Ling Y, Cai L, Yang Y, Liu L, Huang F, Hu Z, Zheng Y. Atmospheric and room temperature plasma mutagenesis of Graesiella emersonii for enhanced protein production using methanol as novel carbon source. Bioresour Technol. 2025;435(132880). https://doi.org/10.1016/j.biortech.2025.132880. |
| [14] |
Zeng Y, Meng W, Hu Y, Peng N, Li J, Zhao S. Mutagenesis and breeding of γ-aminobutyric acid-producing lactic acid bacteria: optimization of fermentation conditions and transcriptome analysis. Food Biosci. 2025;74(107780). https://doi.org/10.1016/j.fbio.2025.107780. |
| [15] |
|
| [16] |
Declerck L, Bouchon F, Demeester W, Guidi C, De Mey M. Accelerated adaptive laboratory evolution: a tool for evolutionary biotechnology. Biotechnol Adv. 2026;86(108741). https://doi.org/10.1016/j.biotechadv.2025.108741. |
| [17] |
|
| [18] |
Tan X, Zhang Q, Liu J, Shang Y, Min Y, Sun X, Tang J. Enhanced γ-aminobutyric acid production by co-culture fermentation with Enterococcus faecium AB157 and Saccharomyces cerevisiae SC125. LWT. 2024;208(116739). https://doi.org/10.1016/j.lwt.2024.116739. |
| [19] |
Park SJ, Kim DH, Kang HJ, Shin M, Yang S, Yang J, Jung YH. Enhanced production of γ-aminobutyric acid (GABA) using Lactobacillus plantarum EJ2014 with simple medium composition. LWT. 2021;137(110443). https://doi.org/10.1016/j.lwt.2020.110443. |
| [20] |
|
| [21] |
Song C, Luo J, Qiao J, Liu Z, Cheng Z, Zhou Z, Han L. pH-adaptive evolution of glutamate decarboxylase enables gamma-aminobutyric acid biosynthesis without pH control. Bioresour Technol. 2026;440(133432). https://doi.org/10.1016/j.biortech.2025.133432. |
| [22] |
Cataldo PG, Villegas JM, Savoy De Giori G, Saavedra L, Hebert EM. Enhancement of γ-aminobutyric acid (GABA) production by Lactobacillus brevis CRL 2013 based on carbohydrate fermentation. Int J Food Microbiol. 2020;333(108792). https://doi.org/10.1016/j.ijfoodmicro.2020.108792. |
| [23] |
|
| [24] |
|
| [25] |
|
| [26] |
|
| [27] |
Su X, Liu M, Lu J, Liu L, Wu K, Bai Y, Li S. Screening of lactic acid bacteria with high γ-aminobutyric acid production and its application in banana juice. J Food Compost Anal. 2025;148(108552). https://doi.org/10.1016/j.jfca.2025.108552. |
| [28] |
Yang L, Zhang X, Chen J, Zhang Y, Feng Z. Expanding the pH range of glutamate decarboxylase from L. pltarum LC84 by site-directed mutagenesis. Front Bioeng Biotechnol. 2023;11(1160818). https://doi.org/10.3389/fbioe.2023.1160818. |
| [29] |
|
Jiangnan University
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