Lignocellulosic fraction-induced niche differentiation within dissimilatory iron reducing bacterial groups in a paddy soil

Highlights

● Responses of soil DIRB to lignocellulosic fractions during a 6-week microcosm incubation were investigated.

● Anaeromyxobacter , Bacillus , and Clostridium maintained their dominance throughout the incubation.

● Distinct DIRB groups proliferated under specific lignocellulosic fraction amendments.

● Limits of insufficient ferric iron on the proliferation varied by DIRB group.

Abstract

Dissimilatory iron reducing bacteria (DIRB) are phylogenetically and physiologically diverse in paddy soils, where iron reduction closely couples with the oxidation of rice straw-derived carbon in the straw returning scenarios. However, few studies have addressed the niche differentiation within DIRB groups during the degradation of lignocellulosic fractions of rice straw. This study conducted a 6-week microcosm incubation experiment to reveal the distinct responses of DIRB groups under specific lignocellulosic fraction amendments with and without ferrihydrite (Fh) addition in a flooded paddy Ultisol. Results showed that the total absolute abundance of the 19 detected DIRB groups did not vary significantly during the incubation. Anaeromyxobacter, Bacillus, and Clostridium were the dominant DIRB groups for all lignocellulosic treatments whereas Thermincola was dominant but only under xylan amendment with Fh addition. DIRB-nodes in the co-occurrence networks of bacterial community mainly belonged to Anaeromyxobacter and Bacillus. Clostridium and Thermincola, Alkaliphilus and Anaeromyxobacter, and Alicyclobacillus, Desulfobulbus, and Desulfosporosinus were specifically proliferated under xylan, cellulose, and lignin amendments, respectively. Whether the proliferation was limited by insufficient ferric iron varied by bacterial group. These findings suggested the lignocellulosic fraction-induced niche differentiation within DIRB groups, which advanced our understanding of the ecology of DIRB in paddy soils under straw returning.

Graphical abstract

Keywords

cellulose / hemicellulose / lignin / bacterial community diversity / co-occurrence networks

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Acknowledgments

This study was funded by the National Natural Science Foundation of China (Grant Nos. 41730753 and 42177033), the Natural Science Foundation of the Higher Education Institutions of Jiangsu Province (Grant No. 21KJB210010), and the Natural Science Foundation of Jiangxi Province (Grant No. 20224BAB203033).

Electronic supplementary material

Supplementary material is available in the online version of this article at https://doi.org/10.1007/s42832-023-0194-2 and is accessible for authorized users.

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