Long-term warming does not affect soil ecoenzyme activity and original microbial nutrient limitation on the Qinghai–Tibet Plateau

Highlights

•No notable effect from long-term warming on activity of nutrient-acquiring enzymes.

•Long-term warming does not notably affect enzymatic stoichiometry.

•Significant, positive correlation between ecoenzyme activity and soil nutrients, microbial biomass.

•Phosphorus limitation found for all soil microbes at different depths.

Abstract

Microbes play an important role in the carbon cycle and nutrient flow of the soil ecosystem. However, the response of microbial activities to long-term warming over decades is poorly understood. To determine how warming changes ecoenzyme activity and microbial nutrient limitation, we conducted a long-term, 21 years, experiment, on the Qinghai–Tibet Plateau. We selected typical grass- and shrub-covered plots, used fiberglass open-top chambers (OTCs) to raise the temperature, conducted soil sampling at different depths, studied the response of nutrient-acquiring enzyme activity and stoichiometry, and conducted vector analysis of stoichiometry. Our results showed that long-term warming did not have a notable effect on the activity of nutrient-acquiring enzymes or enzymatic stoichiometry. However, Spearman correlation analysis indicated a significant and positive correlation between ecoenzyme activity and the available nutrients and microbial biomass in soil. Vector analysis of stoichiometry showed phosphorus limitation for all soil microbes at different depths, regardless of whether the soil experienced warming. These changes in enzymatic stoichiometry and vector analysis suggested that microbial nutrient limitation was not alleviated substantially by long-term warming, and warming did not considerably affect the stratification of microbial nutrient limitation. Our research has also shown that long-term warming does not significantly change soil ecoenzyme activity and original microbial nutrient limitation at different soil depths within the OTUsʼ impact range. These results could help improve understanding of microbial thermal acclimation and response to future long-term global warming.

Graphical abstract

Keywords

Global warming / Ecoenzymatic stoichiometry / Microbial nutrient limitation / Thermal acclimation / Qinghai–Tibet Plateau

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Acknowledgments

This work was supported financially by the National Natural Science Foundation of China (31672475), Qinghai Provincial Key Laboratory of Restoration Ecology in Cold Regions, North-west Institute of Plateau Biology (2020-KF- 04) and Qinghai Innovation Platform Construction Project (2021-ZJ-Y010).

Conflict of interest statement

This article has no conflict of interest and has not been published in other journals.

Electronic supplementary material

Supplementary material is available in the online version of this article at https://doi.org/10.1007/s42832-021-0116-0 and is accessible for authorized users.

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