Protein & Cell >

2023 , Vol. 14 >Issue 4: 304 - 308

DOI: https://doi.org/10.1093/procel/pwac014

LETTER

Enhancing prime editing efficiency and flexibility with tethered and split pegRNAs

  • Ying Feng 1,2 ,
  • Siyuan Liu 2 ,
  • Qiqin Mo 2 ,
  • Pengpeng Liu 3 ,
  • Xiao Xiao , 1 ,
  • Hanhui Ma , 2
Expand
  • 1. School of Biotechnology, East China University of Science and Technology, Shanghai 200237, China
  • 2. Gene Editing Center, School of Life Science and Technology, ShanghaiTech University, Shanghai 200135, China; School of Biotechnology, East China University of Science and Technology, Shanghai 200237, China
  • 3. Department of Molecular, Cell and Cancer Biology, University of Massachusetts Medical School, Worcester, MA 01655, USA
xiaoxiao@ecust.edu.cn
mahh@shanghaitech.edu.cn

Accepted date: 06 Apr 2022

Copyright

2022 ©The Author(s) 2022. Published by Oxford University Press on behalf of Higher Education Press.
Fold

Cite this article

Ying Feng , Siyuan Liu , Qiqin Mo , Pengpeng Liu , Xiao Xiao , Hanhui Ma . Enhancing prime editing efficiency and flexibility with tethered and split pegRNAs[J]. Protein & Cell, 2023 , 14(4) : 304 -308 . DOI: 10.1093/procel/pwac014

References

Publishing order | Descend order by publishing year | Descend order by cited within
1
Anzalone AV, Gao XD, Podracky CJ et al. Programmable deletion, replacement, integration and inversion of large DNA sequences with twin prime editing. Nat Biotechnol 2022;40:731–740.

DOI

2
Anzalone AV, Randolph PB, Davis JR et al. Search-and-replace genome editing without double-strand breaks or donor DNA. Nature 2019;576:149–157.

DOI

3
Bae S, Park J, Kim JS. Cas-OFFinder: a fast and versatile algorithm that searches for potential off-target sites of Cas9 RNA-guided endonucleases. Bioinformatics 2014;30:1473–1475.

DOI

4
Chen PJ, Hussmann JA, Yan J et al. Enhanced prime editing systems by manipulating cellular determinants of editing outcomes. Cell 2021;184:5635–5652.e29.

DOI

5
Choi J, Chen W, Suiter CC et al. Precise genomic deletions using paired prime editing. Nat Biotechnol 2022;40:218–226.

DOI

6
Convery MA, Rowsell S, Stonehouse NJ et al. Crystal structure of an RNA aptamer-protein complex at 2.8 A resolution. Nat Struct Biol 1998;5:133–139.

DOI

7
Kosicki M, Tomberg K, Bradley A. Repair of double-strand breaks induced by CRISPR-Cas9 leads to large deletions and complex rearrangements. Nat Biotechnol 2018;36:765–771.

DOI

8
Landrum MJ, Lee JM, Benson M et al. ClinVar: public archive of interpretations of clinically relevant variants. Nucleic Acids Res 2016;44:D862–D868.

DOI

9
Lin Q, Zong Y, Xue C et al. Prime genome editing in rice and wheat. Nat Biotechnol 2020;38:582–585.

DOI

10
Litke JL, Jaffrey SR. Highly efficient expression of circular RNA aptamers in cells using autocatalytic transcripts. Nat Biotechnol 2019;37:667–675.

DOI

11
Liu Y, Yang G, Huang S et al. Enhancing prime editing by Csy4-mediated processing of pegRNA. Cell Res 2021;31:1134–1136.

DOI

12
Ma H, Tu LC, Naseri A et al. CRISPR-Cas9 nuclear dynamics and target recognition in living cells. J Cell Biol 2016;214:529–537.

DOI

13
Ma H, Tu LC, Naseri A et al. CRISPR-Sirius: RNA scaffolds for signal amplification in genome imaging. Nat Methods 2018;15:928–931.

DOI

14
Nelson JW, Randolph PB, Shen SP et al. Engineered pegRNAs improve prime editing efficiency. Nat Biotechnol 2022;40:402–410.

DOI

15
Stanton BZ, Chory EJ, Crabtree GR. Chemically induced proximity in biology and medicine. Science 2018;359:6380.

DOI

16
Urbanek MO, Galka-Marciniak P, Olejniczak M et al. RNA imaging in living cells—methods and applications. RNA Biol 2014;11:1083–1095.

DOI

Options
Outlines

/

〈 〉