Protein & Cell >

2023 , Vol. 14 >Issue 4: 299 - 303

DOI: https://doi.org/10.1093/procel/pwac018

Letter

Systematic identification of CRISPR off-target effects by CROss-seq

  • Yan Li 1 ,
  • Shengyao Zhi 1 ,
  • Tong Wu 2 ,
  • Hong-Xuan Chen 1 ,
  • Rui Kang 1 ,
  • Dong-Zhao Ma 1 ,
  • Zhou Songyang 1 ,
  • Chuan He , 2,3 ,
  • Puping Liang , 1 ,
  • Guan-Zheng Luo , 1
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  • 1. MOE Key Laboratory of Gene Function and Regulation, Guangdong Province Key Laboratory of Pharmaceutical Functional Genes, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China
  • 2. Department of Chemistry, University of Chicago, Chicago, IL 60637, USA
  • 3. Institute for Biophysical Dynamics, Department of Biochemistry and Molecular Biology, Howard Hughes Medical Institute, University of Chicago, Chicago, IL 60637, USA
chuanhe@uchicago.edu
liangpp5@mail.sysu.edu.cn
luogzh5@mail.sysu.edu.cn

Accepted date: 31 Mar 2022

Copyright

2022 ©The Author(s) 2022. Published by Oxford University Press on behalf of Higher Education Press.
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Cite this article

Yan Li , Shengyao Zhi , Tong Wu , Hong-Xuan Chen , Rui Kang , Dong-Zhao Ma , Zhou Songyang , Chuan He , Puping Liang , Guan-Zheng Luo . Systematic identification of CRISPR off-target effects by CROss-seq[J]. Protein & Cell, 2023 , 14(4) : 299 -303 . DOI: 10.1093/procel/pwac018

References

Publishing order | Descend order by publishing year | Descend order by cited within
1
Anzalone AV, Koblan LW, Liu DR. Genome editing with CRISPR–Cas nucleases, base editors, transposases and prime editors. Nat Biotechnol 2020;38:824–844.

DOI

2
Anzalone AV, Randolph PB, Davis JR et al. Search-and-replace genome editing without double-strand breaks or donor DNA. Nature 2019;576:149–157.

DOI

3
Gaudelli NM, Komor AC, Rees HA et al. Programmable base editing of A·T to G·C in genomic DNA without DNA cleavage. Nature 2017;551:464–471.

DOI

4
Jin S, Lin Q, Luo Y et al. Genome-wide specificity of prime editors in plants. Nat Biotechnol 2021;39:1292–1299.

DOI

5
Kim D, Bae S, Park J et al. Digenome-seq: genome-wide profiling of CRISPR-Cas9 off-target effects in human cells. Nat Methods 2015;12:237–243, 1 p following 243.

DOI

6
Kim D, Kim J. DIG-seq: a genome-wide CRISPR off-target profiling method using chromatin DNA. Genome Res 2018;28:1894–1900.

DOI

7
Kim D, Lim K, Kim S et al. Genome-wide target specificities of CRISPR RNA-guided programmable deaminases. Nat Biotechnol 2017;35:475–480.

DOI

8
Kim DY, Moon SB, Ko J et al. Unbiased investigation of specificities of prime editing systems in human cells. Nucleic Acids Res 2020;48:10576–10589.

DOI

9
Koblan LW, Doman JL, Wilson C et al. Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction. Nat Biotechnol 2018;36:843–846.

DOI

10
Komor AC, Kim YB, Packer MS et al. Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage. Nature 2016;533:420–424.

DOI

11
Liang P, Xie X, Zhi S et al. Genome-wide profiling of adenine base editor specificity by EndoV-seq. Nat Commun 2019;10:1–9.

DOI

12
Richter MF, Zhao KT, Eton E et al. Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity. Nat Biotechnol 2020;38:883–891.

DOI

13
Weng X, Gong J, Chen Y et al. Keth-seq for transcriptome-wide RNA structure mapping. Nat Chem Biol 2020;16:489–492.

DOI

14
Wu T, Lyu R, You Q et al. Kethoxal-assisted single-stranded DNA sequencing captures global transcription dynamics and enhancer activity in situ. Nat Methods 2020;17:515–523.

DOI

15
Zhang H, Li T, Sun Y et al. Perfecting targeting in CRISPR. Annu Rev Genet 2021;55:453–477.

DOI

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