Systematic identification of CRISPR off-target effects by CROss-seq

Yan Li; Shengyao Zhi; Tong Wu; Hong-Xuan Chen; Rui Kang; Dong-Zhao Ma; Zhou Songyang; Chuan He; Puping Liang; Guan-Zheng Luo

doi:10.1093/procel/pwac018

PDF(2709 KB)

Protein Cell ›› 2023, Vol. 14 ›› Issue (4) : 299-303. DOI: 10.1093/procel/pwac018

Letter

Systematic identification of CRISPR off-target effects by CROss-seq

Author information +

History +

Cite this article

EndNote

Ris (Procite)

Bibtex

Download citation ▾

Yan Li, Shengyao Zhi, Tong Wu, Hong-Xuan Chen, Rui Kang, Dong-Zhao Ma, Zhou Songyang, Chuan He, Puping Liang, Guan-Zheng Luo. Systematic identification of CRISPR off-target effects by CROss-seq. Protein Cell, 2023, 14(4): 299‒303 https://doi.org/10.1093/procel/pwac018

References

Publishing order | Descend order by publishing year | Descend order by cited within

[1]	Anzalone AV, Koblan LW, Liu DR. Genome editing with CRISPR–Cas nucleases, base editors, transposases and prime editors. Nat Biotechnol 2020;38:824–844. CrossRef Google scholar

[2]	Anzalone AV, Randolph PB, Davis JR et al. Search-and-replace genome editing without double-strand breaks or donor DNA. Nature 2019;576:149–157. CrossRef Google scholar

[3]	Gaudelli NM, Komor AC, Rees HA et al. Programmable base editing of A·T to G·C in genomic DNA without DNA cleavage. Nature 2017;551:464–471. CrossRef Google scholar

[4]	Jin S, Lin Q, Luo Y et al. Genome-wide specificity of prime editors in plants. Nat Biotechnol 2021;39:1292–1299. CrossRef Google scholar

[5]	Kim D, Bae S, Park J et al. Digenome-seq: genome-wide profiling of CRISPR-Cas9 off-target effects in human cells. Nat Methods 2015;12:237–243, 1 p following 243. CrossRef Google scholar

[6]	Kim D, Kim J. DIG-seq: a genome-wide CRISPR off-target profiling method using chromatin DNA. Genome Res 2018;28:1894–1900. CrossRef Google scholar

[7]	Kim D, Lim K, Kim S et al. Genome-wide target specificities of CRISPR RNA-guided programmable deaminases. Nat Biotechnol 2017;35:475–480. CrossRef Google scholar

[8]	Kim DY, Moon SB, Ko J et al. Unbiased investigation of specificities of prime editing systems in human cells. Nucleic Acids Res 2020;48:10576–10589. CrossRef Google scholar

[9]	Koblan LW, Doman JL, Wilson C et al. Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction. Nat Biotechnol 2018;36:843–846. CrossRef Google scholar

[10]	Komor AC, Kim YB, Packer MS et al. Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage. Nature 2016;533:420–424. CrossRef Google scholar

[11]	Liang P, Xie X, Zhi S et al. Genome-wide profiling of adenine base editor specificity by EndoV-seq. Nat Commun 2019;10:1–9. CrossRef Google scholar

[12]	Richter MF, Zhao KT, Eton E et al. Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity. Nat Biotechnol 2020;38:883–891. CrossRef Google scholar

[13]	Weng X, Gong J, Chen Y et al. Keth-seq for transcriptome-wide RNA structure mapping. Nat Chem Biol 2020;16:489–492. CrossRef Google scholar

[14]	Wu T, Lyu R, You Q et al. Kethoxal-assisted single-stranded DNA sequencing captures global transcription dynamics and enhancer activity in situ. Nat Methods 2020;17:515–523. CrossRef Google scholar