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Structure of intact human MCU supercomplex with the auxiliary MICU subunits

  • Wei Zhuo , 1 ,
  • Heng Zhou 1 ,
  • Runyu Guo 1 ,
  • Jingbo Yi 1 ,
  • Laixing Zhang 1 ,
  • Lei Yu 1 ,
  • Yinqiang Sui 1 ,
  • Wenwen Zeng 2,3 ,
  • Peiyi Wang , 4 ,
  • Maojun Yang , 1
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  • 1. Ministry of Education Key Laboratory of Protein Science, Tsinghua-Peking Joint Center for Life Sciences, Beijing Advanced Innovation Center for Structural Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China
  • 2. Institute for Immunology and School of Medicine, Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing 100084, China
  • 3. Beijing Key Laboratory for Immunological Research on Chronic Diseases, Tsinghua University, Beijing 100084, China
  • 4. Cryo-EM Facility Center, Southern University of Science Technology, Shenzhen 518055, China

Published date: 15 Mar 2021

Copyright

2020 The Author(s) 2020

Cite this article

Wei Zhuo , Heng Zhou , Runyu Guo , Jingbo Yi , Laixing Zhang , Lei Yu , Yinqiang Sui , Wenwen Zeng , Peiyi Wang , Maojun Yang . Structure of intact human MCU supercomplex with the auxiliary MICU subunits[J]. Protein & Cell, 2021 , 12(3) : 220 -229 . DOI: 10.1007/s13238-020-00776-w

1
Csordas G, Golenar T, Seifert EL, Kamer KJ, Sancak Y, Perocchi F, Moffat C, Weaver D, Perez SD, Bogorad R (2013) MICU1 controls both the threshold and cooperative activation of the mitochondrial Ca2+ uniporter. Cell Metab 17:976–987

DOI

2
De Stefani D, Rizzuto R, Pozzan T (2016) Enjoy the trip: calcium in mitochondria back and forth. Annu Rev Biochem 85:161–192

DOI

3
Fan M, Zhang J, Tsai CW, Orlando BJ, Rodriguez M, Xu Y, Liao M, Tsai MF, Feng L (2020) Structure and mechanism of the mitochondrial Ca(2+) uniporter holocomplex. Nature 582:129–133

DOI

4
Hoffman NE, Chandramoorthy HC, Shamugapriya S, Zhang X, Rajan S, Mallilankaraman K, Gandhirajan RK, Vagnozzi RJ, Ferrer LM, Sreekrishnanilayam K (2013) MICU1 motifs define mitochondrial calcium uniporter binding and activity. Cell Rep 5:1576–1588

DOI

5
Kamer KJ, Mootha VK (2014) MICU1 and MICU2 play nonredundant roles in the regulation of the mitochondrial calcium uniporter. EMBO Rep 15:299–307

DOI

6
Kirichok Y, Krapivinsky G, Clapham DE (2004) The mitochondrial calcium uniporter is a highly selective ion channel. Nature 427:360–364

DOI

7
Perocchi F, Gohil VM, Girgis HS, Bao XR, McCombs JE, Palmer AE, Mootha VK (2010) MICU1 encodes a mitochondrial EF hand protein required for Ca2+ uptake. Nature 467:291–U267

DOI

8
Phillips CB, Tsai CW, Tsai MF (2019) The conserved aspartate ring of MCU mediates MICU1 binding and regulation in the mitochondrial calcium uniporter complex. Elife 8.

DOI

9
Tsai MF, Phillips CB, Ranaghan M, Tsai CW, Wu Y, Willliams C, Miller C (2016) Dual functions of a small regulatory subunit in the mitochondrial calcium uniporter complex. Elife 5.

DOI

10
Vasington FD, Murphy JV (1962) Ca++ uptake by rat kidney mitochondria and its dependence on respiration and phosphorylation. J Biol Chem 237:2670–000

11
Vivek G, Ishan P, Tiffany U, Junji S, Lorin SM, Yuriy K (2020) The mechanism of MICU-dependent gating of the mitochondrial Ca2+ uniporter. bioRxiv 2020.04.04.025833.

12
Wang L, Yang X, Li S, Wang Z, Liu Y, Feng J, Zhu Y, Shen Y (2014) Structural and mechanistic insights into MICU1 regulation of mitochondrial calcium uptake. EMBO J 33:594–604

DOI

13
Wang Y, Nguyen NX, She J, Zeng W, Yang Y, Bai XC, Jiang Y (2019) Structural mechanism of EMRE-dependent gating of the human mitochondrial calcium uniporter. Cell 177(1252–1261):e1213

DOI

14
Yamamoto T, Yamagoshi R, Harada K, Kawano M, Minami N, Ido Y, Kuwahara K, Fujita A, Ozono M, Watanabe A (2016) Analysis of the structure and function of EMRE in a yeast expression system. Biochim Biophys Acta 1857:831–839

DOI

15
Yamamoto T, Ozono M, Watanabe A, Maeda K, Nara A, Hashida M, Ido Y, Hiroshima Y, Yamada A, Terada H (2019) Functional analysis of coiled-coil domains of MCU in mitochondrial calcium uptake. Biochim Biophys Acta Bioenergy 148061

DOI

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