Structure of intact human MCU supercomplex with the auxiliary MICU subunits

Wei Zhuo, Heng Zhou, Runyu Guo, Jingbo Yi, Laixing Zhang, Lei Yu, Yinqiang Sui, Wenwen Zeng, Peiyi Wang, Maojun Yang

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Protein Cell ›› 2021, Vol. 12 ›› Issue (3) : 220-229. DOI: 10.1007/s13238-020-00776-w
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Structure of intact human MCU supercomplex with the auxiliary MICU subunits

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Wei Zhuo, Heng Zhou, Runyu Guo, Jingbo Yi, Laixing Zhang, Lei Yu, Yinqiang Sui, Wenwen Zeng, Peiyi Wang, Maojun Yang. Structure of intact human MCU supercomplex with the auxiliary MICU subunits. Protein Cell, 2021, 12(3): 220‒229 https://doi.org/10.1007/s13238-020-00776-w

References

[1]
Csordas G, Golenar T, Seifert EL, Kamer KJ, Sancak Y, Perocchi F, Moffat C, Weaver D, Perez SD, Bogorad R (2013) MICU1 controls both the threshold and cooperative activation of the mitochondrial Ca2+ uniporter. Cell Metab 17:976–987
CrossRef Google scholar
[2]
De Stefani D, Rizzuto R, Pozzan T (2016) Enjoy the trip: calcium in mitochondria back and forth. Annu Rev Biochem 85:161–192
CrossRef Google scholar
[3]
Fan M, Zhang J, Tsai CW, Orlando BJ, Rodriguez M, Xu Y, Liao M, Tsai MF, Feng L (2020) Structure and mechanism of the mitochondrial Ca(2+) uniporter holocomplex. Nature 582:129–133
CrossRef Google scholar
[4]
Hoffman NE, Chandramoorthy HC, Shamugapriya S, Zhang X, Rajan S, Mallilankaraman K, Gandhirajan RK, Vagnozzi RJ, Ferrer LM, Sreekrishnanilayam K (2013) MICU1 motifs define mitochondrial calcium uniporter binding and activity. Cell Rep 5:1576–1588
CrossRef Google scholar
[5]
Kamer KJ, Mootha VK (2014) MICU1 and MICU2 play nonredundant roles in the regulation of the mitochondrial calcium uniporter. EMBO Rep 15:299–307
CrossRef Google scholar
[6]
Kirichok Y, Krapivinsky G, Clapham DE (2004) The mitochondrial calcium uniporter is a highly selective ion channel. Nature 427:360–364
CrossRef Google scholar
[7]
Perocchi F, Gohil VM, Girgis HS, Bao XR, McCombs JE, Palmer AE, Mootha VK (2010) MICU1 encodes a mitochondrial EF hand protein required for Ca2+ uptake. Nature 467:291–U267
CrossRef Google scholar
[8]
Phillips CB, Tsai CW, Tsai MF (2019) The conserved aspartate ring of MCU mediates MICU1 binding and regulation in the mitochondrial calcium uniporter complex. Elife 8.
CrossRef Google scholar
[9]
Tsai MF, Phillips CB, Ranaghan M, Tsai CW, Wu Y, Willliams C, Miller C (2016) Dual functions of a small regulatory subunit in the mitochondrial calcium uniporter complex. Elife 5.
CrossRef Google scholar
[10]
Vasington FD, Murphy JV (1962) Ca++ uptake by rat kidney mitochondria and its dependence on respiration and phosphorylation. J Biol Chem 237:2670–000
[11]
Vivek G, Ishan P, Tiffany U, Junji S, Lorin SM, Yuriy K (2020) The mechanism of MICU-dependent gating of the mitochondrial Ca2+ uniporter. bioRxiv 2020.04.04.025833.
[12]
Wang L, Yang X, Li S, Wang Z, Liu Y, Feng J, Zhu Y, Shen Y (2014) Structural and mechanistic insights into MICU1 regulation of mitochondrial calcium uptake. EMBO J 33:594–604
CrossRef Google scholar
[13]
Wang Y, Nguyen NX, She J, Zeng W, Yang Y, Bai XC, Jiang Y (2019) Structural mechanism of EMRE-dependent gating of the human mitochondrial calcium uniporter. Cell 177(1252–1261):e1213
CrossRef Google scholar
[14]
Yamamoto T, Yamagoshi R, Harada K, Kawano M, Minami N, Ido Y, Kuwahara K, Fujita A, Ozono M, Watanabe A (2016) Analysis of the structure and function of EMRE in a yeast expression system. Biochim Biophys Acta 1857:831–839
CrossRef Google scholar
[15]
Yamamoto T, Ozono M, Watanabe A, Maeda K, Nara A, Hashida M, Ido Y, Hiroshima Y, Yamada A, Terada H (2019) Functional analysis of coiled-coil domains of MCU in mitochondrial calcium uptake. Biochim Biophys Acta Bioenergy 148061
CrossRef Google scholar

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