LETTER

Dynamic cell transition and immune response landscapes of axolotl limb regeneration revealed by single-cell analysis

  • Hanbo Li 1,2 ,
  • Xiaoyu Wei 1,3,4 ,
  • Li Zhou 1,2 ,
  • Weiqi Zhang 5,6,7 ,
  • Chen Wang 1,2 ,
  • Yang Guo 2 ,
  • Denghui Li 2 ,
  • Jianyang Chen 2 ,
  • Tianbin Liu 1,4,8 ,
  • Yingying Zhang 2 ,
  • Shuai Ma 6,7,9 ,
  • Congyan Wang 2 ,
  • Fujian Tan 2 ,
  • Jiangshan Xu 1,3,4 ,
  • Yang Liu 1,3,4 ,
  • Yue Yuan 1,3,4 ,
  • Liang Chen 10 ,
  • Qiaoran Wang 5,6 ,
  • Jing Qu 6,7,11 ,
  • Yue Shen 1,4,8 ,
  • Shanshan Liu 1,2 ,
  • Guangyi Fan 1,2 ,
  • Longqi Liu 1,4 ,
  • Xin Liu 1,2 ,
  • Yong Hou 1,4 ,
  • Guang-Hui Liu , 6,7,12 ,
  • Ying Gu , 1,4,8 ,
  • Xun Xu , 1,4,7,8,13
Expand
  • 1. BGI-Shenzhen, Shenzhen 518083, China
  • 2. BGI-Qingdao, Qingdao 266555, China
  • 3. BGI Education Center, University of Chinese Academy of Sciences, Shenzhen 518083, China
  • 4. China National Gene Bank, BGI-Shenzhen, Shenzhen 518120, China
  • 5. CAS Key Laboratory of Genomic and Precision Medicine, Beijing Institute of Genomics, China National Center for Bioinformation, Chinese Academy of Sciences, Beijing 100101, China
  • 6. University of Chinese Academy of Sciences, Beijing 100049, China
  • 7. Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing 100101, China
  • 8. Guangdong Provincial Key Laboratory of Genome Read and Write, BGI-Shenzhen, Shenzhen 518083, China
  • 9. National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
  • 10. Hubei Key Laboratory of Cell Homeostasis, College of Life Sciences, Wuhan University, Wuhan 40072, China
  • 11. State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China
  • 12. State Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China
  • 13. Synthetic Biology Technology Innovation Center of Shandong Province, Qingdao 266003, China

Published date: 15 Jan 2021

Copyright

2020 The Author(s) 2020

Cite this article

Hanbo Li , Xiaoyu Wei , Li Zhou , Weiqi Zhang , Chen Wang , Yang Guo , Denghui Li , Jianyang Chen , Tianbin Liu , Yingying Zhang , Shuai Ma , Congyan Wang , Fujian Tan , Jiangshan Xu , Yang Liu , Yue Yuan , Liang Chen , Qiaoran Wang , Jing Qu , Yue Shen , Shanshan Liu , Guangyi Fan , Longqi Liu , Xin Liu , Yong Hou , Guang-Hui Liu , Ying Gu , Xun Xu . Dynamic cell transition and immune response landscapes of axolotl limb regeneration revealed by single-cell analysis[J]. Protein & Cell, 2021 , 12(1) : 57 -66 . DOI: 10.1007/s13238-020-00763-1

1
Doyle SL, O'Neill LA (2006) Toll-like receptors: from the discovery of NFkappaB to new insights into transcriptional regulations in innate immunity. Biochem Pharmacol 72:1102–1113

DOI

2
Erickson JR, Gearhart MD, Honson DD, Reid TA, Gardner MK, Moriarity BS, Echeverri K (2016) A novel role for SALL4 during scar-free wound healing in axolotl. NPJ Regener Med 1:1–11

DOI

3
Forte E, Chimenti I, Rosa P, Angelini F, Pagano F, Calogero A, Giacomello A, Messina E (2017) EMT/MET at the crossroad of stemness, regeneration and oncogenesis: he Ying-Yang equilibrium recapitulated in cell spheroids. Cancers 9:98

DOI

4
Gerber T, Murawala P, Knapp D, Masselink W, Schuez M, Hermann S, Gac-Santel M, Nowoshilow S, Kageyama J, Khattak (2018). Single-cell analysis uncovers convergence of cell identities during axolotl limb regeneration. Science (New York, NY) 362

DOI

5
Godwin JW, Pinto AR, Rosenthal NA (2013) Macrophages are required for adult salamander limb regeneration. Proc Natl Acad Sci USA 110:9415–9420

DOI

6
Haas BJ, Whited JL (2017) Advances in decoding axolotl limb regeneration. Trends Genet 33:553–565

DOI

7
Kalluri R, Weinberg RA (2009) The basics of epithelial-mesenchymal transition. J Clin Investig 119:1420–1428

DOI

8
Leigh ND, Dunlap GS, Johnson K, Mariano R, Oshiro R, Wong AY, Bryant DM, Miller BM, Ratner A, Chen A (2018) Transcriptomic landscape of the blastema niche in regenerating adult axolotl limbs at single-cell resolution. Nat Commun 9:5153

DOI

9
Marwick JA, Mills R, Kay O, Michail K, Stephen J, Rossi AG, Dransfield I, Hirani N (2018) Neutrophils induce macrophage antiinflammatory reprogramming by suppressing NF-κB activation. Cell Death Dis 9:665

DOI

10
Meng XM, Nikolic-Paterson DJ, Lan HY (2016) TGF-beta: the master regulator of fibrosis. Nat Rev Nephrol 12:325–338

DOI

11
Rayagiri SS, Ranaldi D, Raven A, Mohamad Azhar NIF, Lefebvre O, Zammit PS, Borycki AG (2018) Basal lamina remodeling at the skeletal muscle stem cell niche mediates stem cell self-renewal. Nat Commun 9:1075

DOI

12
Sharif O, Bolshakov VN, Raines S, Newham P, Perkins ND (2007) Transcriptional profiling of the LPS induced NF-kappaB response in macrophages. BMC Immunol 8:1

DOI

13
Stone RC, Pastar I, Ojeh N, Chen V, Liu S, Garzon KI, Tomic-Canic M (2016) Epithelial-mesenchymal transition in tissue repair and fibrosis. Cell Tissue Res 365:495–506

DOI

14
Wynn TA, Vannella KM (2016) Macrophages in tissue repair, regeneration, and fibrosis. Immunity 44:450–462

DOI

15
Zhang L, Huang G, Li X, Zhang Y, Jiang Y, Shen J, Liu J, Wang Q, Zhu J, Feng X (2013) Hypoxia induces epithelial-mesenchymal transition via activation of SNAI1 by hypoxia-inducible factor-1alpha in hepatocellular carcinoma. BMC Cancer 13:108

DOI

Outlines

/