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Structure of African swine fever virus p15 reveals its dual role for membrane-association and DNA binding

  • Dan Fu 1 ,
  • Dongming Zhao 2 ,
  • Wei Zhang 1 ,
  • Guangshun Zhang 1 ,
  • Mingyu Li 1 ,
  • Zheng Zhang 1 ,
  • Yuhui Wang 1 ,
  • Dongdong Sun 1 ,
  • Peng Jiao 1 ,
  • Cheng Chen , 3 ,
  • Yu Guo , 1 ,
  • Zihe Rao 1
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  • 1. State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy, Nankai University, Tianjin 300350, People’s Republic of China
  • 2. State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, People’s Republic of China
  • 3. School of Life Sciences, Tianjin University, Tianjin 300071, People’s Republic of China

Published date: 15 Aug 2020

Copyright

2020 The Author(s)

Cite this article

Dan Fu , Dongming Zhao , Wei Zhang , Guangshun Zhang , Mingyu Li , Zheng Zhang , Yuhui Wang , Dongdong Sun , Peng Jiao , Cheng Chen , Yu Guo , Zihe Rao . Structure of African swine fever virus p15 reveals its dual role for membrane-association and DNA binding[J]. Protein & Cell, 2020 , 11(8) : 606 -612 . DOI: 10.1007/s13238-020-00731-9

1
Andres G, Alejo A, Salas J, Salas ML (2002) African swine fever virus polyproteins pp220 and pp62 assemble into the core shell. J Virol 76:12473–12482

DOI

2
Andres G, Charro D, Matamoros T, Dillard RS, Abrescia NGA (2020) The cryo-EM structure of African swine fever virus unravels a unique architecture comprising two icosahedral protein capsids and two lipoprotein membranes. J Biol Chem 295:1–12

DOI

3
Andres G, Garcia-Escudero R, Vinuela E, Salas ML, Rodriguez JM (2001) African swine fever virus structural protein pE120R is essential for virus transport from assembly sites to plasma membrane but not for infectivity. J Virol 75:6758–6768

DOI

4
Hill CP, Worthylake D, Bancroft DP, Christensen AM, Sundquist WI (1996) Crystal structures of the trimeric human immunodeficiency virus type 1 matrix protein: implications for membrane association and assembly. Proc Natl Acad Sci USA 93:3099–3104

DOI

5
Holm L, Sander C (1995) Dali: a network tool for protein structure comparison. Trends Biochem Sci 20:478–480

DOI

6
Iyer LM, Aravind L, Koonin EV (2001) Common origin of four diverse families of large eukaryotic DNA viruses. J Virol 75:11720–11734

DOI

7
Liu S, Luo Y, Wang Y, Li S, Zhao Z, Bi Y, Sun J, Peng R, Song H, Zhu D (2019) Cryo-EM structure of the African swine fever virus. Cell Host Microbe 26(836–843):e833

DOI

8
Rao Z, Belyaev AS, Fry E, Roy P, Jones IM, Stuart DI (1995) Crystal structure of SIV matrix antigen and implications for virus assembly. Nature 378:743–747

DOI

9
Ruigrok RW, Barge A, Durrer P, Brunner J, Ma K, Whittaker GR (2000) Membrane interaction of influenza virus M1 protein. Virology 267:289–298

DOI

10
Seifert GJ (2018) Fascinating fasciclins: a surprisingly widespread family of proteins that mediate interactions between the cell exterior and the cell surface. Int J Mol Sci 19:E1628

DOI

11
Sha B, Luo M (1997) Structure of a bifunctional membrane-RNA binding protein, influenza virus matrix protein M1. Nat Struct Biol 4:239–244

DOI

12
Simon-Mateo C, Andres G, Almazan F, Vinuela E (1997) Proteolytic processing in African swine fever virus: evidence for a new structural polyprotein, pp62. J Virol 71:5799–5804

DOI

13
Simon-Mateo C, Andres G, Vinuela E (1993) Polyprotein processing in African swine fever virus: a novel gene expression strategy for a DNA virus. EMBO J 12:2977–2987

DOI

14
Suarez C, Salas ML, Rodriguez JM (2010) African swine fever virus polyprotein pp62 is essential for viral core development. J Virol 84:176–187

DOI

15
Wang N, Zhao D, Wang J, Zhang Y, Wang M, Gao Y, Li F, Wang J, Bu Z, Rao Z (2019) Architecture of African swine fever virus and implications for viral assembly. Science 366:640–644

DOI

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