Protein & Cell >

2019 , Vol. 10 >Issue 11: 832 - 839

DOI: https://doi.org/10.1007/s13238-019-0611-6

LETTER

Multiple sgRNAs facilitate base editingmediated i-stop to induce complete and precise gene disruption

  • Kun Jia 1 ,
  • Zongyang Lu 3 ,
  • Fei Zhou 2 ,
  • Zhiqi Xiong 3 ,
  • Rui Zhang 1 ,
  • Zhiwei Liu 2 ,
  • Yu’e Ma 2 ,
  • Lei He 2 ,
  • Cong Li 2 ,
  • Zhen Zhu 4 ,
  • Dejing Pan , 2 ,
  • Zhengxing Lian , 1
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  • 1. Beijing Key Laboratory of Animal Genetic Improvement, China Agricultural University, Beijing 100094, China
  • 2. Cambridge-Suda Genomic Resource Center and Jiangsu Key Laboratory of Neuropsychiatric Diseases Research, Soochow University, Suzhou 215123, China
  • 3. School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China
  • 4. Key Laboratory of MEMS of Ministry of Education, Southeast University, Nanjing 210096, China

Published date: 15 Nov 2019

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2019 The Author(s) 2019
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Cite this article

Kun Jia , Zongyang Lu , Fei Zhou , Zhiqi Xiong , Rui Zhang , Zhiwei Liu , Yu’e Ma , Lei He , Cong Li , Zhen Zhu , Dejing Pan , Zhengxing Lian . Multiple sgRNAs facilitate base editingmediated i-stop to induce complete and precise gene disruption[J]. Protein & Cell, 2019 , 10(11) : 832 -839 . DOI: 10.1007/s13238-019-0611-6

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