LETTER

In vitro transcribed sgRNA causes cell death by inducing interferon release

  • Wei Mu 1,2 ,
  • Na Tang 1,2 ,
  • Chen Cheng 1,3 ,
  • Wen Sun 1 ,
  • Xiaofei Wei 4 ,
  • Haoyi Wang , 1,2,5
Expand
  • 1. State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China
  • 2. University of Chinese Academy of Sciences, Beijing 100049, China
  • 3. School of Life Sciences, University of Science and Technology of China, Hefei 230026, China
  • 4. Beijing Cord Blood Bank, Beijing 100176, China
  • 5. Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing 100101, China

Published date: 15 Jun 2019

Copyright

2019 The Author(s)

Cite this article

Wei Mu , Na Tang , Chen Cheng , Wen Sun , Xiaofei Wei , Haoyi Wang . In vitro transcribed sgRNA causes cell death by inducing interferon release[J]. Protein & Cell, 2019 , 10(6) : 461 -465 . DOI: 10.1007/s13238-018-0605-9

1
Cheng AW, Jillette N, Lee P, Plaskon D, Fujiwara Y, Wang W, Taghbalout A, Wang H (2016) Casilio: a versatile CRISPR-Cas9-Pumilio hybrid for gene regulation and genomic labeling. Cell Res 26:254–257

DOI

2
Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA (2013) Multiplex genome engineering using CRISPR/Cas systems. Science 339:819–823

DOI

3
Dever DP, Bak RO, Reinisch A, Camarena J, Washington G, Nicolas CE, Pavel-Dinu M, Saxena N, Wilkens AB, Mantri S (2016) CRISPR/Cas9 beta-globin gene targeting in human hematopoietic stem cells. Nature 539:384–389

DOI

4
Dong-Ho K, Longo M, Young H, Lundberg P, Cantin E, Rossi JJ (2004) Interferon induction by siRNAs and ssRNAs synthesized by phage polymerase. Nat Biotechnol 22:321

DOI

5
Gundry MC, Brunetti L, Lin A, Mayle AE, Kitano A, Wagner D, Hsu JI, Hoegenauer KA, Rooney C, Goodell MA (2016) Highly efficient genome editing of murine and human hematopoietic progenitor cells by CRISPR/Cas9. Cell Rep 17:1453–1461

DOI

6
Hendel A, BakR O, Clark JT, Kennedy AB, Ryan DE, Roy S, Steinfeld I, Lunstad BD, Kaiser RJ, Wilkens AB (2015) Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cells. Nat Biotechnol 33:985

DOI

7
Kim S, Kim D, Cho SW, Kim J, Kim J-S (2014) Highly efficient RNAguided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins. Genome Res 24:1012–1019

DOI

8
Kim S, Koo T, Jee H-G, Cho H-Y, Lee G, Lim D-G, Shin HS, Kim J-S (2018) CRISPR RNAs trigger innate immune responses in human cells. Genome Res 28:367–373

DOI

9
Liu X, Zhang Y, Cheng C, Cheng AW, Zhang X, Li N, Xia C, Wei X, Liu X, Wang H (2016) CRISPR-Cas9-mediated multiplex gene editing in CAR-T cells. Cell Res 27:154

DOI

10
Mali P, Yang L, Esvelt KM, Aach J, Guell M, DiCarlo JE, Norville JE, Church GM (2013) RNA-guided human genome engineering via Cas9. Science 339:823–826

DOI

11
Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F (2013) Genome engineering using the CRISPR-Cas9 system. Nat Protoc 8:2281–2308

DOI

12
Ren J, Zhang X, Liu X, Fang C, Jiang S, June CH, Zhao Y (2017) A versatile system for rapid multiplex genome-edited CAR T cell generation. Oncotarget 8:17002–17011

DOI

13
Schumann K, Lin S, Boyer E, Simeonov DR, Subramaniam M, Gate RE, Haliburton GE, Ye CJ, Bluestone JA, Doudna JA (2015) Generation of knock-in primary human T cells using Cas9 ribonucleoproteins. Proc Natl Acad Sci USA 112:10437–10442

DOI

14
Shechner DM, Hacisuleyman E, Younger ST, Rinn JL (2015) Multiplexable, locus-specific targeting of long RNAs with CRISPR-Display. Nat Methods 12:664–670

DOI

15
Zhang Y, Mu W, Wang H (2017) Gene editing in T cell therapy. J Genet Genomics 44:415–422

DOI

Outlines

/