Protein & Cell >

2018 , Vol. 9 >Issue 8: 733 - 737

DOI: https://doi.org/10.1007/s13238-017-0468-5

LETTER

APEX2- tagging of Sigma 1-receptor indicates subcellular protein topology with cytosolic N-terminus and ER luminal C-terminus

  • Timur Mavylutov 1 ,
  • Xi Chen 1 ,
  • Lianwang Guo 2 ,
  • Jay Yang , 1
Expand
  • 1. Department of Anesthesiology, University of Wisconsin SMPH, Madison, WI 53705, USA
  • 2. Department of Surgery and Physiology & Cell Biology, Ohio State University, Columbus, OH 43210, USA

Published date: 14 Aug 2018

Copyright

2017 The Author(s) 2017. This article is an open access publication
Fold

Cite this article

Timur Mavylutov , Xi Chen , Lianwang Guo , Jay Yang . APEX2- tagging of Sigma 1-receptor indicates subcellular protein topology with cytosolic N-terminus and ER luminal C-terminus[J]. Protein & Cell, 2018 , 9(8) : 733 -737 . DOI: 10.1007/s13238-017-0468-5

References

Publishing order | Descend order by publishing year | Descend order by cited within
1
Aydar E, Palmer CP,Klyachko VA,Jackson MB (2002) The sigma receptor as a ligand-regulated auxiliary potassium channel subunit. Neuron 34:399–410

DOI

2
Dudek J, Pfeffer S, Lee PH, Jung M, Cavalie A, Helms V, Forster F, Zimmermann R (2015) Protein transport into the human endoplasmic reticulum. J Mol Biol 427:1159–1175

DOI

3
Hayashi T, Su T-P (2003) Sigma1 receptors (sigma binding sites) form raft-like microdomains and target lipid droplets on the endoplasmic reticulum: roles in endoplasmic reticulum lipid compartmentalization and export. J Pharmacol Exp Therap 306:718–725

DOI

4
Hayashi T, Su T-P (2007) Sigma-1 receptor chaperones at the ERmitochondrion interface regulate Ca(2+) signaling and cell survival. Cell 131:596–610

DOI

5
Lam S, Martell JD, Kamer KJ, Deerinck TJ, Ellisman MH, Mootha VK, Ting AY (2015) Direct evolution of APEX2 for electron microscopyo and proximity labeling. Nature Meth 12:51–54

DOI

6
Lee S, Kang M, Park J,Lee G,Alice Y, Lee S, Kang M, Park J,Lee G,Ting AY, Rhee H (2016) Resource APEX fingerprinting reveals the subcellular localization of proteins of interest. Cell Rep 15:1837–1847

DOI

7
Mason MRJ, Ehlert EME, Eggers R, Pool CW, Hermening S, Huseinovic A, Timmermans E, Blits B, Verhaagen J (2010) Comparison of AAV serotypes for gene delivery to dorsal root ganglion neurons. Mol Ther 18:715–724

DOI

8
Mavlyutov TA, Epstein ML, Andersen KA, Ziskind-Conhaim L, Ruoho AE (2010) The sigma-1 receptor is enriched in postsynaptic sites of C-terminals in mouse motoneurons. An anatomical and behavioral study. Neuroscience 167:247–255

DOI

9
Mavlyutov TA, Epstein M, Guo L-W(2015) Subcellular localization of the sigma-1 receptor in retinal neurons- an electron microscopy study. Sci Rep 5:1–11

DOI

10
Mavlyutov TA, Duellman T, Kim HT, Epstein ML, Leese C, Davletov BA, Yang J (2016) Sigma-1 receptor expression in the dorsal root ganglion: reexamination using a highly specific antibody. Neuroscience 331:148–157

DOI

11
Mavlyutov TA, Yang H, Epstein ML, Ruoho AE, Yang J (2017) APEX2-enhanced electron microscopy distinguishes sigma-1 receptor localization in the nucleoplasmic reticulum. Oncotarget.

DOI

12
Rhee HW, Zou P, Udeshi NM, Martell JD, Mootha VK, Carr SA, Tine AY (2013) Proteomic mapping of mitochondria in living cells via spatially restricted enzymatic tagging. Science 339:1328–1331

DOI

13
Schmidt HR, Zheng S, Gurpinar E, Koehl A, Manglik A, Kruse AC (2016) Crystal structure of the human σ1 receptor. Nature 532:527–530

DOI

14
Storek B, Reinhardt M, Wang C,Janssen WGM, Harder NM, Banck MS, Morrison JH, Beutler AS (2008) Sensory neuron targeting by self-complimentary AAV8 via lumbar puncture for chronic pain. Proc Natl Acad Sci USA 209:1055–1060

DOI

15
Su TP, Su TC, Nakamura Y, Tsai SY (2016) The sigma-1 receptor as a pluripotent modulator in living systems. Trends Pharmacol Sci 37:262–278

DOI

Options
Outlines

/

〈 〉