APEX2- tagging of Sigma 1-receptor indicates subcellular protein topology with cytosolic N-terminus and ER luminal C-terminus

References

[1] Aydar E, Palmer CP,Klyachko VA,Jackson MB (2002) The sigma receptor as a ligand-regulated auxiliary potassium channel subunit. Neuron 34:399–410

[2] Dudek J, Pfeffer S, Lee PH, Jung M, Cavalie A, Helms V, Forster F, Zimmermann R (2015) Protein transport into the human endoplasmic reticulum. J Mol Biol 427:1159–1175

[3] Hayashi T, Su T-P (2003) Sigma1 receptors (sigma binding sites) form raft-like microdomains and target lipid droplets on the endoplasmic reticulum: roles in endoplasmic reticulum lipid compartmentalization and export. J Pharmacol Exp Therap 306:718–725

[4] Hayashi T, Su T-P (2007) Sigma-1 receptor chaperones at the ERmitochondrion interface regulate Ca(2+) signaling and cell survival. Cell 131:596–610

[5] Lam S, Martell JD, Kamer KJ, Deerinck TJ, Ellisman MH, Mootha VK, Ting AY (2015) Direct evolution of APEX2 for electron microscopyo and proximity labeling. Nature Meth 12:51–54

[6] Lee S, Kang M, Park J,Lee G,Alice Y, Lee S, Kang M, Park J,Lee G,Ting AY, Rhee H (2016) Resource APEX fingerprinting reveals the subcellular localization of proteins of interest. Cell Rep 15:1837–1847

[7] Mason MRJ, Ehlert EME, Eggers R, Pool CW, Hermening S, Huseinovic A, Timmermans E, Blits B, Verhaagen J (2010) Comparison of AAV serotypes for gene delivery to dorsal root ganglion neurons. Mol Ther 18:715–724

[8] Mavlyutov TA, Epstein ML, Andersen KA, Ziskind-Conhaim L, Ruoho AE (2010) The sigma-1 receptor is enriched in postsynaptic sites of C-terminals in mouse motoneurons. An anatomical and behavioral study. Neuroscience 167:247–255

[9] Mavlyutov TA, Epstein M, Guo L-W(2015) Subcellular localization of the sigma-1 receptor in retinal neurons- an electron microscopy study. Sci Rep 5:1–11

[10] Mavlyutov TA, Duellman T, Kim HT, Epstein ML, Leese C, Davletov BA, Yang J (2016) Sigma-1 receptor expression in the dorsal root ganglion: reexamination using a highly specific antibody. Neuroscience 331:148–157

[11] Mavlyutov TA, Yang H, Epstein ML, Ruoho AE, Yang J (2017) APEX2-enhanced electron microscopy distinguishes sigma-1 receptor localization in the nucleoplasmic reticulum. Oncotarget.

[12] Rhee HW, Zou P, Udeshi NM, Martell JD, Mootha VK, Carr SA, Tine AY (2013) Proteomic mapping of mitochondria in living cells via spatially restricted enzymatic tagging. Science 339:1328–1331

[13] Schmidt HR, Zheng S, Gurpinar E, Koehl A, Manglik A, Kruse AC (2016) Crystal structure of the human σ1 receptor. Nature 532:527–530

[14] Storek B, Reinhardt M, Wang C,Janssen WGM, Harder NM, Banck MS, Morrison JH, Beutler AS (2008) Sensory neuron targeting by self-complimentary AAV8 via lumbar puncture for chronic pain. Proc Natl Acad Sci USA 209:1055–1060

[15] Su TP, Su TC, Nakamura Y, Tsai SY (2016) The sigma-1 receptor as a pluripotent modulator in living systems. Trends Pharmacol Sci 37:262–278

RIGHTS & PERMISSIONS

The Author(s) 2017. This article is an open access publication