Protein & Cell >

2017 , Vol. 8 >Issue 10: 776 - 779

DOI: https://doi.org/10.1007/s13238-017-0458-7

LETTER

Highly efficient and precise base editing in discarded human tripronuclear embryos

  • Guanglei Li 1 ,
  • Yajing Liu 2 ,
  • Yanting Zeng 1 ,
  • Jianan Li 2 ,
  • Lijie Wang 2 ,
  • Guang Yang 2 ,
  • Dunjin Chen 1,4 ,
  • Xiaoyun Shang 3 ,
  • Jia Chen 2 ,
  • Xingxu Huang , 2 ,
  • Jianqiao Liu , 1
Expand
  • 1. Department of Reproductive Medicine, Third Affiliated Hospital of Guangzhou Medical University, Guangzhou 510150, China
  • 2. School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China
  • 3. Institute of Immunology, PLA, Third Military Medical University, Chongqing 400038, China
  • 4. Key Laboratory for Reproduction and Genetics of Guangdong Higher Education Institutes, Key Laboratory for Major Obstetric Diseases of Guangdong Province, Third Affiliated Hospital of Guangzhou Medical University, Guangzhou 510150, China & Correspondence huangxx@shanghaitech.edu.cn (X. Huang), liujqssz@gzhmu.edu.cn (J. Liu)

Published date: 06 Nov 2017

Copyright

2017 The Author(s) 2017. This article is an open access publication
Fold

Cite this article

Guanglei Li , Yajing Liu , Yanting Zeng , Jianan Li , Lijie Wang , Guang Yang , Dunjin Chen , Xiaoyun Shang , Jia Chen , Xingxu Huang , Jianqiao Liu . Highly efficient and precise base editing in discarded human tripronuclear embryos[J]. Protein & Cell, 2017 , 8(10) : 776 -779 . DOI: 10.1007/s13238-017-0458-7

References

Publishing order | Descend order by publishing year | Descend order by cited within
1
CallawayE (2016) Embryo-editing research gathers momentum. Nature532:289–290

DOI

2
CyranoskiD, ReardonS (2015) Embryo editing sparks epic debate. Nature520:593–594

DOI

3
KangX, HeW, HuangY, YuQ, ChenY, GaoX, SunX, FanY (2016) Introducing precise genetic modifications into human 3PN embryos by CRISPR/Cas-mediated genome editing. J Assist Reprod Genet33:581–588

DOI

4
KomorAC, KimYB, PackerMS, ZurisJA, LiuDR (2016) Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage. Nature533:420–424

DOI

5
KomorAC, BadranAH, LiuDR (2017) CRISPR-based technologies for the manipulation of eukaryotic genomes. Cell168:20–36

DOI

6
LiangP, XuY, ZhangX, DingC, HuangR, ZhangZ, LvJ, XieX, ChenY, LiY (2015) CRISPR/Cas9-mediated gene editing in human tripronuclear zygotes. Protein Cell6:363–372

DOI

7
Plaza ReyesA, LannerF (2017) Towards a CRISPR view of early human development: applications, limitations and ethical concerns of genome editing in human embryos. Development144:3–7

DOI

8
ShenB, ZhangW, ZhangJ, ZhouJ, WangJ, ChenL, WangL, HodgkinsA, IyerV, HuangX (2014) Efficient genome modification by CRISPR-Cas9 nickase with minimal off-target effects. Nat Methods11:399–402

DOI

9
TangL, ZengY, DuH, GongM, PengJ, ZhangB, LeiM, ZhaoF, WangW, LiX (2017) CRISPR/Cas9-mediated gene editing in human zygotes using Cas9 protein. Mol Genet Genomics292:525–533

DOI

10
TsaiSQ, JoungJK (2016) Defining and improving the genome-wide specificities of CRISPR-Cas9 nucleases. Nat Rev Genet17:300–312

DOI

11
TsaiSQ, ZhengZ, NguyenNT, LiebersM, TopkarVV, ThaparV, WyvekensN, KhayterC, IafrateAJ, LeLP (2015) GUIDEseq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases. Nat Biotechnol33:187–197

DOI

Options
Outlines

/

〈 〉