Highly efficient and precise base editing in discarded human tripronuclear embryos

Guanglei Li; Yajing Liu; Yanting Zeng; Jianan Li; Lijie Wang; Guang Yang; Dunjin Chen; Xiaoyun Shang; Jia Chen; Xingxu Huang; Jianqiao Liu

doi:10.1007/s13238-017-0458-7

PDF(495 KB)

Protein Cell ›› 2017, Vol. 8 ›› Issue (10) : 776-779. DOI: 10.1007/s13238-017-0458-7

LETTER

Highly efficient and precise base editing in discarded human tripronuclear embryos

Author information +

History +

Cite this article

EndNote

Ris (Procite)

Bibtex

Download citation ▾

Guanglei Li, Yajing Liu, Yanting Zeng, Jianan Li, Lijie Wang, Guang Yang, Dunjin Chen, Xiaoyun Shang, Jia Chen, Xingxu Huang, Jianqiao Liu. Highly efficient and precise base editing in discarded human tripronuclear embryos. Protein Cell, 2017, 8(10): 776‒779 https://doi.org/10.1007/s13238-017-0458-7

References

Publishing order | Descend order by publishing year | Descend order by cited within

[1]	CallawayE (2016) Embryo-editing research gathers momentum. Nature532:289–290 CrossRef Google scholar

[2]	CyranoskiD, ReardonS (2015) Embryo editing sparks epic debate. Nature520:593–594 CrossRef Google scholar

[3]	KangX, HeW, HuangY, YuQ, ChenY, GaoX, SunX, FanY (2016) Introducing precise genetic modifications into human 3PN embryos by CRISPR/Cas-mediated genome editing. J Assist Reprod Genet33:581–588 CrossRef Google scholar

[4]	KomorAC, KimYB, PackerMS, ZurisJA, LiuDR (2016) Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage. Nature533:420–424 CrossRef Google scholar

[5]	KomorAC, BadranAH, LiuDR (2017) CRISPR-based technologies for the manipulation of eukaryotic genomes. Cell168:20–36 CrossRef Google scholar

[6]	LiangP, XuY, ZhangX, DingC, HuangR, ZhangZ, LvJ, XieX, ChenY, LiY (2015) CRISPR/Cas9-mediated gene editing in human tripronuclear zygotes. Protein Cell6:363–372 CrossRef Google scholar

[7]	Plaza ReyesA, LannerF (2017) Towards a CRISPR view of early human development: applications, limitations and ethical concerns of genome editing in human embryos. Development144:3–7 CrossRef Google scholar

[8]	ShenB, ZhangW, ZhangJ, ZhouJ, WangJ, ChenL, WangL, HodgkinsA, IyerV, HuangX (2014) Efficient genome modification by CRISPR-Cas9 nickase with minimal off-target effects. Nat Methods11:399–402 CrossRef Google scholar

[9]	TangL, ZengY, DuH, GongM, PengJ, ZhangB, LeiM, ZhaoF, WangW, LiX (2017) CRISPR/Cas9-mediated gene editing in human zygotes using Cas9 protein. Mol Genet Genomics292:525–533 CrossRef Google scholar

[10]	TsaiSQ, JoungJK (2016) Defining and improving the genome-wide specificities of CRISPR-Cas9 nucleases. Nat Rev Genet17:300–312 CrossRef Google scholar

[11]	TsaiSQ, ZhengZ, NguyenNT, LiebersM, TopkarVV, ThaparV, WyvekensN, KhayterC, IafrateAJ, LeLP (2015) GUIDEseq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases. Nat Biotechnol33:187–197 CrossRef Google scholar