Protein & Cell >

2017 , Vol. 8 >Issue 10: 772 - 775

DOI: https://doi.org/10.1007/s13238-017-0459-6

LETTER

Highly efficient base editing in human tripronuclear zygotes

  • Changyang Zhou 1,2 ,
  • Meiling Zhang 3 ,
  • Yu Wei 1 ,
  • Yidi Sun 2,5,6,7 ,
  • Yun Sun 3 ,
  • Hong Pan 1,8 ,
  • Ning Yao 3 ,
  • Wanxia Zhong 3 ,
  • Yixue Li 2,5,6,7 ,
  • Weiping Li , 3 ,
  • Hui Yang , 1 ,
  • Zi-jiang Chen , 3,4
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  • 1. Institute of Neuroscience, State Key Laboratory of Neuroscience, Key Laboratory of Primate Neurobiology, CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China
  • 2. College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China
  • 3. Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics, Center for Reproductive Medicine, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China
  • 4. Center for Reproductive Medicine, Shandong Provincial Hospital Affiliated to Shandong University, National Research Center for Assisted Reproductive Technology and Reproductive Genetics, The Key laboratory for Reproductive Endocrinology of Ministry of Education, Jinan 250021, China
  • 5. Key Lab of Computational Biology, CAS-MPG Partner Institute for Computational Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China
  • 6. Institute of Biomedical Sciences, Fudan University, Medical College, Shanghai 200032, China
  • 7. Shanghai Center for Bioinformation Technology, Shanghai Industrial Technology Institute, Shanghai 201203, China
  • 8. State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresource, Guangxi University, Nanning 530004, China

Published date: 06 Nov 2017

Copyright

2017 The Author(s) 2017. This article is an open access publication
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Cite this article

Changyang Zhou , Meiling Zhang , Yu Wei , Yidi Sun , Yun Sun , Hong Pan , Ning Yao , Wanxia Zhong , Yixue Li , Weiping Li , Hui Yang , Zi-jiang Chen . Highly efficient base editing in human tripronuclear zygotes[J]. Protein & Cell, 2017 , 8(10) : 772 -775 . DOI: 10.1007/s13238-017-0459-6

References

Publishing order | Descend order by publishing year | Descend order by cited within
1
KangX, HeW, HuangY, YuQ, ChenY, GaoX, SunX, FanY(2016) Introducing precise genetic modifications into human 3PN embryos by CRISPR/Cas-mediated genome editing. J Assist Reprod Genet33:581–588

DOI

2
KimK, RyuSM, KimST, BaekG, KimD, LimK, ChungE, KimS, KimJS (2017a) Highly efficient RNA-guided base editing in mouse embryos. Nat Biotechnol35:435–437

DOI

3
KimYB, KomorAC, LevyJM, PackerMS, ZhaoKT, LiuDR (2017b) Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions. Nat Biotechnol35:371–376

DOI

4
KomorAC, KimYB, PackerMS, ZurisJA, LiuDR (2016) Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage. Nature533:420–424

DOI

5
LiangP, SunH, SunY, ZhangX, XieX, ZhangJ, ZhangZ, ChenY, DingC, XiongY(2017) Effective gene editing by high-fidelity base editor 2 in mouse zygotes. Protein Cell8(8):601–611

DOI

6
LiangP, XuY, ZhangX, DingC, HuangR, ZhangZ, LvJ, XieX, ChenY, LiY (2015) CRISPR/Cas9-mediated gene editing in human tripronuclear zygotes. Protein Cell6:363–372

DOI

7
LongC, McAnallyJR, SheltonJM, MireaultAA, Bassel-DubyR, OlsonEN (2014) Prevention of muscular dystrophy in mice by CRISPR/Cas9-mediated editing of germline DNA. Science345:1184–1188

DOI

8
MaY, ZhangJ, YinW, ZhangZ, SongY, ChangX (2016) Targeted AID-mediated mutagenesis (TAM) enables efficient genomic diversification in mammalian cells. Nat Methods13:1029–1035

DOI

9
NishidaK, ArazoeT, YachieN, BannoS, KakimotoM, TabataM, MochizukiM, MiyabeA, ArakiM, HaraKY (2016) Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems. Science 353:aaf8729

DOI

10
TangL, ZengY, DuH, GongM, PengJ, ZhangB, LeiM, ZhaoF, WangW, LiX (2017) CRISPR/Cas9-mediated gene editing in human zygotes using Cas9 protein. Mol Genet Genom292:525–533

DOI

11
WangH, YangH, ShivalilaCS, DawlatyMM, ChengAW, ZhangF, JaenischR (2013) One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated genome engineering. Cell153:910–918

DOI

12
WuY, LiangD, WangY, BaiM, TangW, BaoS, YanZ, LiD, LiJ (2013) Correction of a genetic disease in mouse via use of CRISPR-Cas9. Cell Stem Cell13:659–662

DOI

13
YangH, WangH, ShivalilaCS, ChengAW, ShiL, JaenischR(2013) One-step generation of mice carrying reporter and conditional alleles by CRISPR/Cas-mediated genome engineering. Cell154:1370–1379

DOI

14
ZongY, WangY, LiC, ZhangR, ChenK, RanY, QiuJL, WangD, GaoC (2017) Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion. Nat Biotechnol35:438–440

DOI

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