Protein & Cell >

2015 , Vol. 06 >Issue 12: 919 - 923

DOI: https://doi.org/10.1007/s13238-015-0219-4

LETTER

An improved method for phasing crystal structures with low non-crystallographic symmetry using cryo-electron microscopy data

  • Jia Wang 1,2,3 ,
  • Weiguang Wang 1,2 ,
  • Wen Song 2,3,4 ,
  • Zhifu Han 2,3,4 ,
  • Heqiao Zhang 2,3,4 ,
  • Jijie Chai , 2,3,4 ,
  • Hongwei Wang , 2,3,4 ,
  • Jiawei Wang , 1,2
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  • 1. State Key Laboratory of Membrane Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China
  • 2. Center for Structural Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China
  • 3. Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing 100084, China
  • 4. Ministry of Education Key Laboratory of Protein Science, School of Life Sciences, Tsinghua University, Beijing 100084, China

Published date: 04 Jan 2016

Copyright

2014 This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Cite this article

Jia Wang , Weiguang Wang , Wen Song , Zhifu Han , Heqiao Zhang , Jijie Chai , Hongwei Wang , Jiawei Wang . An improved method for phasing crystal structures with low non-crystallographic symmetry using cryo-electron microscopy data[J]. Protein & Cell, 2015 , 06(12) : 919 -923 . DOI: 10.1007/s13238-015-0219-4

References

Publishing order | Descend order by publishing year | Descend order by cited within
1
Abrescia NG, Grimes JM, Oksanen HM, Bamford JK, Bamford DH, Stuart D I (2011) The use of low-resolution phasing followed by phase extension from 7.6 to 2.5 A resolution with noncrystallographic symmetry to solve the structure of a bacteriophage capsid protein. Acta Crystallogr D Biol Crystallogr 67:228–232

DOI

2
Anderson DH, Kickhoefer VA, Sievers SA, Rome LH, Eisenberg D (2007) Draft crystal structure of the vault shell at 9-A resolution. PLoS Biol 5:e318

3
Dodson EJ (2001) Using electron-microscopy images as a model for molecular replacement. Acta Crystallogr D Biol Crystallogr 57:1405–1409

DOI

4
Jenni S, Ban N (2009) Imperfect pseudo-merohedral twinning in crystals of fungal fatty acid synthase. Acta Crystallogr D Biol Crystallogr 65:101–111

DOI

5
Kuhlbrandt W (2014) Cryo-EM enters a new era. eLife 3:e03678

6
McCoy AJ, Grosse-Kunstleve RW, Adams PD, Winn MD, Storoni LC, Read RJ (2007) Phaser crystallographic software. J Appl Crystallogr 40:658–674

DOI

7
Navaza J (2008) Combining X-ray and electron-microscopy data to solve crystal structures. Acta Crystallogr D Biol Crystallogr 64:70–75

DOI

8
Nemecek D, Plevka P, Boura E (2013) Using cryoEM reconstruction and phase extension to determine crystal structure of bacteriophage varphi6 major capsid protein. Protein J 32:635–640

DOI

9
Read RJ (1986) Improved Fourier coefficients for maps using phases from partial structures with errors. Acta Crystallogr Sect A 42:140–149

DOI

10
Rossmann MG (1972) The molecular replacement method: a collection of papers on the use of non-crystallographic symmetry, vol 13. Routledge, London

11
Song W, Wang J, Han Z, Zhang Y, Zhang H, Wang W, Chang J, Xia B, Fan S, Zhang D. (2015) Structural basis for specific recognition of single-stranded RNA by toll-like receptor 13. Nat Struct Mol Biol 22:782–787

DOI

12
Stuart DI, Abrescia NG (2013) From lows to highs: using lowresolution models to phase X-ray data. Acta Crystallogr D Biol Crystallogr 69:2257–2265

DOI

13
Terwilliger TC (2000) Maximum-likelihood density modification. Acta Crystallogr D Biol Crystallogr 56:965–972

DOI

14
Trapani S, Schoehn G, Navaza J, Abergel C (2010) Macromolecular crystal data phased by negative-stained electron-microscopy reconstructions. Acta Crystallogr D Biol Crystallogr 66:514–521

DOI

15
Xiong Y (2008) From electron microscopy to X-ray crystallography: molecular-replacement case studies. Acta Crystallogr D Biol Crystallogr 64:76–82

DOI

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