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Nano-size uni-lamellar lipodisq improved in situ auto-phosphorylation analysis of E. coli tyrosine kinase using 19F nuclear magnetic resonance

  • Dong Li 1 ,
  • Juan Li 1 ,
  • Yonglong Zhuang 3 ,
  • Longhua Zhang 1 ,
  • Ying Xiong 1 ,
  • Pan Shi , 2 ,
  • Changlin Tian , 1,2
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  • 1. Hefei National Laboratory for Physical Science at the Microscale School of Life Science, University of Science and Technology of China, Hefei 230026, China
  • 2. High Magnetic Field Laboratory, Chinese Academy of Sciences, Hefei 230031, China
  • 3. Department of Chemistry, Anhui University, Hefei 230027, China

Published date: 01 Apr 2015

Copyright

2014 This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Cite this article

Dong Li , Juan Li , Yonglong Zhuang , Longhua Zhang , Ying Xiong , Pan Shi , Changlin Tian . Nano-size uni-lamellar lipodisq improved in situ auto-phosphorylation analysis of E. coli tyrosine kinase using 19F nuclear magnetic resonance[J]. Protein & Cell, 2015 , 6(3) : 229 -233 . DOI: 10.1007/s13238-014-0129-x

1
Barnes CO, Pielak GJ (2011) In-cell protein NMR and protein leakage. Proteins79: 347-351

DOI

2
Bayburt TH, Sligar SG (2003) Self-assembly of single integral membrane proteins into soluble nanoscale phospholipid bilayers. Protein Sci12: 2476-2481

DOI

3
Bayburt TH, Sligar SG (2010) Membrane protein assembly into Nanodiscs. FEBS Lett584: 1721-1727

DOI

4
Hammill JT, Miyake-Stoner S, Hazen JL, Jackson JC, Mehl RA (2007) Preparation of site-speciflcally labeled fluorinated proteins for 19F-NMR structural characterization. Nat Protoc2: 2601-2607

DOI

5
Hunter T (2009) Tyrosine phosphorylation: thirty years and counting. Curr Opin Cell Biol21: 140-146

DOI

6
Jackson JC, Hammill JT, Mehl RA (2007) Site-speciflc incorporation of a (19) F-amino acid into proteins as an NMR probe for characterizing protein structure and reactivity. J Am Chem Soc129: 1160-1166

DOI

7
Johnson H, White FM (2012) Toward quantitative phosphotyrosine proflling in vivo. Semin Cell Dev Biol23: 854-862

DOI

8
Knowles TJ, Finka R, Smith C, Lin YP, Dafforn T, Overduin M (2009) Membrane proteins solubilized intact in lipid containing nanoparticles bounded by styrene maleic acid copolymer. J Am Chem Soc131: 7484-7485

DOI

9
Lee DC, Zheng J, She YM, Jia Z (2008) Structure of Escherichia coli tyrosine kinase Etk reveals a novel activation mechanism. EMBO J27: 1758-1766

DOI

10
Li F, Shi P, Li J, Yang F, Wang T, Zhang W, Gao F, Ding W, Li D, Li J (2013) A genetically encoded 19F NMR probe for tyrosine phosphorylation. Angew Chem Int Ed Engl52: 3958-3962

DOI

11
Orwick MC, Judge PJ, Procek J, Lindholm L, Graziadei A, Engel A, Grobner G, Watts A (2012) Detergent-free formation and physicochemical characterization of nanosized lipid-polymer complexes: Lipodisq. Angew Chem Int Ed Engl51: 4653-4657

DOI

12
Orwick-Rydmark M, Lovett JE, Graziadei A, Lindholm L, Hicks MR, Watts A (2012) Detergent-Free incorporation of a seven-trans-membrane receptor protein into nanosized bilayer Lipodisq particles for functional and biophysical studies. Nano lett12: 4687-4692

DOI

13
Raschle T, Hiller S, Etzkorn M, Wagner G (2010) Nonmicellar systems for solution NMR spectroscopy of membrane proteins. Curr Opin Struct Biol20: 471-479

DOI

14
Shi P, Xi Z, Wang H, Shi C, Xiong Y, Tian C (2010) Site-speciflc protein backbone and side-chain NMR chemical shift and relaxation analysis of human vinexin SH3 domain using a genetically encoded 15N/19F-labeled unnatural amino acid. Biochem Biophys Res Commun402: 461-466

DOI

15
Shi P, Wang H, Xi Z, Shi C, Xiong Y, Tian C (2011) Site-speciflc (1)(9) F NMR chemical shift and side chain relaxation analysis of a membrane protein labeled with an unnatural amino acid. Protein Sci20: 224-228

DOI

16
Shi P, Li D, Chen H, Xiong Y, Wang Y, Tian C (2012) In situ 19F NMR studies of an E. coli membrane protein. Protein Sci21: 596-600

DOI

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