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Nano-size uni-lamellar lipodisq improved in situ auto-phosphorylation analysis of E. coli tyrosine kinase using 19F nuclear magnetic resonance
Dong Li, Juan Li, Yonglong Zhuang, Longhua Zhang, Ying Xiong, Pan Shi, Changlin Tian
Protein Cell ›› 2015, Vol. 6 ›› Issue (3) : 229-233.
Nano-size uni-lamellar lipodisq improved in situ auto-phosphorylation analysis of E. coli tyrosine kinase using 19F nuclear magnetic resonance
[1] |
Barnes CO, Pielak GJ (2011) In-cell protein NMR and protein leakage. Proteins79: 347-351
CrossRef
Google scholar
|
[2] |
Bayburt TH, Sligar SG (2003) Self-assembly of single integral membrane proteins into soluble nanoscale phospholipid bilayers. Protein Sci12: 2476-2481
CrossRef
Google scholar
|
[3] |
Bayburt TH, Sligar SG (2010) Membrane protein assembly into Nanodiscs. FEBS Lett584: 1721-1727
CrossRef
Google scholar
|
[4] |
Hammill JT, Miyake-Stoner S, Hazen JL, Jackson JC, Mehl RA (2007) Preparation of site-speciflcally labeled fluorinated proteins for 19F-NMR structural characterization. Nat Protoc2: 2601-2607
CrossRef
Google scholar
|
[5] |
Hunter T (2009) Tyrosine phosphorylation: thirty years and counting. Curr Opin Cell Biol21: 140-146
CrossRef
Google scholar
|
[6] |
Jackson JC, Hammill JT, Mehl RA (2007) Site-speciflc incorporation of a (19) F-amino acid into proteins as an NMR probe for characterizing protein structure and reactivity. J Am Chem Soc129: 1160-1166
CrossRef
Google scholar
|
[7] |
Johnson H, White FM (2012) Toward quantitative phosphotyrosine proflling in vivo. Semin Cell Dev Biol23: 854-862
CrossRef
Google scholar
|
[8] |
Knowles TJ, Finka R, Smith C, Lin YP, Dafforn T, Overduin M (2009) Membrane proteins solubilized intact in lipid containing nanoparticles bounded by styrene maleic acid copolymer. J Am Chem Soc131: 7484-7485
CrossRef
Google scholar
|
[9] |
Lee DC, Zheng J, She YM, Jia Z (2008) Structure of Escherichia coli tyrosine kinase Etk reveals a novel activation mechanism. EMBO J27: 1758-1766
CrossRef
Google scholar
|
[10] |
Li F, Shi P, Li J, Yang F, Wang T, Zhang W, Gao F, Ding W, Li D, Li J
CrossRef
Google scholar
|
[11] |
Orwick MC, Judge PJ, Procek J, Lindholm L, Graziadei A, Engel A, Grobner G, Watts A (2012) Detergent-free formation and physicochemical characterization of nanosized lipid-polymer complexes: Lipodisq. Angew Chem Int Ed Engl51: 4653-4657
CrossRef
Google scholar
|
[12] |
Orwick-Rydmark M, Lovett JE, Graziadei A, Lindholm L, Hicks MR, Watts A (2012) Detergent-Free incorporation of a seven-trans-membrane receptor protein into nanosized bilayer Lipodisq particles for functional and biophysical studies. Nano lett12: 4687-4692
CrossRef
Google scholar
|
[13] |
Raschle T, Hiller S, Etzkorn M, Wagner G (2010) Nonmicellar systems for solution NMR spectroscopy of membrane proteins. Curr Opin Struct Biol20: 471-479
CrossRef
Google scholar
|
[14] |
Shi P, Xi Z, Wang H, Shi C, Xiong Y, Tian C (2010) Site-speciflc protein backbone and side-chain NMR chemical shift and relaxation analysis of human vinexin SH3 domain using a genetically encoded 15N/19F-labeled unnatural amino acid. Biochem Biophys Res Commun402: 461-466
CrossRef
Google scholar
|
[15] |
Shi P, Wang H, Xi Z, Shi C, Xiong Y, Tian C (2011) Site-speciflc (1)(9) F NMR chemical shift and side chain relaxation analysis of a membrane protein labeled with an unnatural amino acid. Protein Sci20: 224-228
CrossRef
Google scholar
|
[16] |
Shi P, Li D, Chen H, Xiong Y, Wang Y, Tian C (2012) In situ 19F NMR studies of an E. coli membrane protein. Protein Sci21: 596-600
CrossRef
Google scholar
|
/
〈 |
|
〉 |