MiR-139-5p inhibits migration and invasion of colorectal cancer by downregulating AMFR and NOTCH1
Received date: 14 Jun 2014
Accepted date: 14 Jul 2014
Published date: 20 Nov 2014
Copyright
MicroRNAs (miRNAs) that exert function by posttranscriptional suppression have recently brought insight in our understanding of the role of non-protein-coding RNAs in carcinogenesis and metastasis. In this study, we described the function and molecular mechanism of miR-139-5p in colorectal cancer (CRC) and its potential clinical application in CRC. We found that miR-139-5p was significantly downregulated in 73.8% CRC samples compared with adjacent noncancerous tissues (NCTs), and decreased miR-139-5p was associated with poor prognosis. Functional analyses demonstrated that ectopic expression of miR-139-5p suppressed CRC cell migration and invasion in vitro and metastasis in vivo. Mechanistic investigations revealed that miR-139-5p suppress CRC cell invasion and metastasis by targeting AMFR and NOTCH1. Knockdown of the two genes phenocopied the inhibitory effect of miR-139-5p on CRC metastasis. Furthermore, the protein levels of the two genes were upregulated in CRC samples compared with NCTs, and inversely correlated with the miR-139-5p expression. Increased NOTCH1 protein expression was correlated with poor prognosis of CRC patients. Together, our data indicate that miR-139-5p is a potential tumor suppressor and prognostic factor for CRC, and targeting miR-139-5p may repress the metastasis of CRC and improve survival.
Key words: miR-139-5p; AMFR; NOTCH1; colorectal cancer; metastasis; prognosis
Mingxu Song , Yuan Yin , Jiwei Zhang , Binbin Zhang , Zehua Bian , Chao Quan , Leyuan Zhou , Yaling Hu , Qifeng Wang , Shujuan Ni , Bojian Fei , Weili Wang , Xiang Du , Dong Hua , Zhaohui Huang . MiR-139-5p inhibits migration and invasion of colorectal cancer by downregulating AMFR and NOTCH1[J]. Protein & Cell, 2014 , 5(11) : 851 -861 . DOI: 10.1007/s13238-014-0093-5
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