Protein & Cell >

2014 , Vol. 5 >Issue 4: 258 - 260

DOI: https://doi.org/10.1007/s13238-014-0032-5

LETTER

Homologous recombination in human embryonic stem cells using CRISPR/Cas9 nickase and a long DNA donor template

  • Zhili Rong 2 ,
  • Shengyun Zhu 1 ,
  • Yang Xu 2 ,
  • Xuemei Fu , 1,3
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  • 1. Shenzhen Children’s Hospital, 7019 Yitian Road, Shenzhen 518026, China
  • 2. Division of Biological Sciences, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA
  • 3. Chongqing Medical University, Chongqing 400016, China

Published date: 24 Jun 2014

Copyright

2014 This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Cite this article

Zhili Rong , Shengyun Zhu , Yang Xu , Xuemei Fu . Homologous recombination in human embryonic stem cells using CRISPR/Cas9 nickase and a long DNA donor template[J]. Protein & Cell, 2014 , 5(4) : 258 -260 . DOI: 10.1007/s13238-014-0032-5

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