Preparation of fatty acid solutions for investigating lipid signaling, metabolism, and lipid droplets

Shuyan Zhang; Mengwei Zhang; Shimeng Xu; Xiaochuan Fu; Qiumin Liao; Bin Pan; Liujuan Cui; Pingsheng Liu

doi:10.1093/procel/pwae068

Protein Cell ›› 2025, Vol. 16 ›› Issue (7) : 609 -614. DOI: 10.1093/procel/pwae068

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Preparation of fatty acid solutions for investigating lipid signaling, metabolism, and lipid droplets

Shuyan Zhang ¹^,²^,³^,⁴^,⁵^,^†
, Mengwei Zhang ⁵^,⁶^,⁷
, Shimeng Xu ⁵
, Xiaochuan Fu ⁵
, Qiumin Liao ¹^,²^,³^,⁴
, Bin Pan ⁵
, Liujuan Cui ⁵
, Pingsheng Liu ⁵^,⁷^,^†

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Shuyan Zhang, Mengwei Zhang, Shimeng Xu, Xiaochuan Fu, Qiumin Liao, Bin Pan, Liujuan Cui, Pingsheng Liu. Preparation of fatty acid solutions for investigating lipid signaling, metabolism, and lipid droplets. Protein Cell, 2025, 16(7): 609-614 DOI:10.1093/procel/pwae068

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References

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[1]	Abumrad N, Harmon C, Ibrahimi A. Membrane transport of long-chain fatty acids: evidence for a facilitated process. J Lipid Res 1998;39:2309–2318.

[2]	Alsabeeh N, Chausse B, Kakimoto PA et al. Cell culture models of fatty acid overload: problems and solutions. Biochim Biophys Acta Mol Cell Biol Lipids 2018;1863:143–151.

[3]	Chang TY, Chang CC, Ohgami N et al. Cholesterol sensing, trafficking, and esterification. Annu Rev Cell Dev Biol 2006;22:129–157.

[4]	Hassain M, Azzazy E, Christenson RH. All about albumin: biochemistry, genetics, and medical applications. Theodore Peters, Jr. San Diego, CA: Academic Press, 1996, 432 pp, $85.00. ISBN 0-12-552110-3. Clin Chem 1997;43:2014a–2015.

[5]	Jiang H, Zhang X, Chen X et al. Protein lipidation: occurrence, mechanisms, biological functions, and enabling technologies. Chem Rev 2018;118:919–988.

[6]	Khan I, Hussain M, Jiang B et al. Omega-3 long-chain polyunsaturated fatty acids: metabolism and health implications. Prog Lipid Res 2023;92:101255.

[7]	Liu P, Ying Y, Zhao Y et al. Chinese hamster ovary K2 cell lipid droplets appear to be metabolic organelles involved in membrane traffic. J Biol Chem 2004;279:3787–3792.

[8]	Nelson DL, Lehninger AL, Cox MM. Lehninger Principles of Biochemistry. W. H. Freeman, New York, 2008.

[9]	O’Byrne SM, Blaner WS. Retinol and retinyl esters: biochemistry and physiology. J Lipid Res 2013;54:1731–1743.

[10]	Oliveira AF, Cunha DA, Ladriere L et al. In vitro use of free fatty acids bound to albumin: a comparison of protocols. Biotechniques 2015;58:228–233.

[11]	Samovski D, Jacome-Sosa M, Abumrad NA. Fatty acid transport and signaling: mechanisms and physiological implications. Annu Rev Physiol 2023;85:317–337.