Quantitative real-time in vitro transcription assay (QRIVTA) for transcriptional regulation studies

Fan Liu , Jing Xu , Xinli Hu , Bo Duan , Bin Xia

Protein Cell ›› 2025, Vol. 16 ›› Issue (6) : 484 -490.

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Protein Cell ›› 2025, Vol. 16 ›› Issue (6) : 484 -490. DOI: 10.1093/procel/pwae054
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Quantitative real-time in vitro transcription assay (QRIVTA) for transcriptional regulation studies

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Fan Liu, Jing Xu, Xinli Hu, Bo Duan, Bin Xia. Quantitative real-time in vitro transcription assay (QRIVTA) for transcriptional regulation studies. Protein Cell, 2025, 16(6): 484-490 DOI:10.1093/procel/pwae054

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References

[1]

Cambray G, Guimaraes JC, Mutalik VK et al. Measurement and modeling of intrinsic transcription terminators. Nucleic Acids Res 2013;41:5139–5148.

[2]

Chen YJ, Liu P, Nielsen AA et al. Characterization of 582 natural and synthetic terminators and quantification of their design constraints. Nat Methods 2013;10:659–664.

[3]

Chong S, Chen C, Ge H et al. Mechanism of transcriptional bursting in bacteria. Cell 2014;158:314–326.

[4]

Dong J, Wu T, Xiao Y et al. Target-triggered transcription machinery for ultra-selective and sensitive fluorescence detection of nucleoside triphosphates in one minute. Biosens Bioelectron 2018;100:333–340.

[5]

Höfer K, Langejürgen LV, Jäschke A. Universal aptamer-based real-time monitoring of enzymatic RNA synthesis. J Am Chem Soc 2013;135:13692–13694.

[6]

Huang YH, Trapp V, Puro O et al. Fluorogenic RNA aptamers to probe transcription initiation and co-transcriptional RNA folding by multi-subunit RNA polymerases. Methods Enzymol 2022;675:207–233.

[7]

Jensen D, Ruiz Manzano A, Rector M et al. High-throughput, fluorescent-aptamer-based measurements of steady-state transcription rates for the Mycobacterium tuberculosis RNA polymerase. Nucleic Acids Res 2023;51:e99.

[8]

Lukose B, Maruno T, Faidh MA et al. Molecular and thermodynamic determinants of self-assembly and hetero-oligomerization in the enterobacterial thermo-osmo-regulatory protein H-NS. Nucleic Acids Res 2024;52:2157–2173.

[9]

Mairhofer J, Wittwer A, Cserjan-Puschmann M et al. Preventing T7 RNA polymerase read-through transcription-A synthetic termination signal capable of improving bioprocess stability. ACS Synth Biol 2015;4:265–273.

[10]

Marras SA, Gold B, Kramer FR et al. Real-time measurement of in vitro transcription. Nucleic Acids Res 2004;32:e72.

[11]

Qin W, Li L, Yang F et al. High-throughput iSpinach fluorescent aptamer-based real-time monitoring of in vitro transcription. Bioresour Bioprocess 2022;9:112.

[12]

Shin M. The mechanism underlying Ler-mediated alleviation of gene repression by H-NS. Biochem Biophys Res Commun 2017;483:392–396.

[13]

Shin M, Lagda AC, Lee JW et al. Gene silencing by H-NS from distal DNA site. Mol Microbiol 2012;86:707–719.

[14]

Will WR, Bale DH, Reid PJ et al. Evolutionary expansion of a regulatory network by counter-silencing. Nat Commun 2014;5:5270.

[15]

Yang X, Ma C. In vitro transcription assays and their application in drug discovery. J Vis Exp 2016;115:e54256.

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The Author(s) 2024. Published by Oxford University Press on behalf of Higher Education Press.

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