CRISPR-based screening pinpoints H2AZ1 as a driver of senescence in human mesenchymal stem cells

Ming-Heng Li; Xiaoyu Jiang; Yaobin Jing; Kaowen Yan; Shi-Jia Bi; Si Wang; Shuai Ma; Guang-Hui Liu; Weiqi Zhang; Shuhui Sun; Jing Qu

doi:10.1093/procel/pwae035

Protein Cell ›› 2025, Vol. 16 ›› Issue (4) : 293 -299. DOI: 10.1093/procel/pwae035

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CRISPR-based screening pinpoints H2AZ1 as a driver of senescence in human mesenchymal stem cells

Ming-Heng Li ¹^,³^,⁵^,⁶^,⁷
, Xiaoyu Jiang ²^,³^,⁵^,⁶^,⁷
, Yaobin Jing ²^,³^,¹⁰
, Kaowen Yan ²^,³^,⁵^,⁶^,⁷
, Shi-Jia Bi ¹^,³^,⁵^,⁶^,⁷
, Si Wang ⁸^,⁹^,¹¹
, Shuai Ma ²^,³^,⁵^,⁶^,⁷^,¹¹
, Guang-Hui Liu ²^,³^,⁵^,⁶^,⁷^,⁸^,⁹^,¹⁰^,¹¹^,^†
, Weiqi Zhang ⁴^,⁵^,⁶^,¹¹^,^†
, Shuhui Sun ²^,³^,⁵^,⁷^,¹²^,^†
, Jing Qu ¹^,²^,³^,⁵^,⁶^,⁷^,¹¹^,¹²^,^†

Author information +

¹. State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China

². State Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China

³. Key Laboratory of Organ Regeneration and Reconstruction, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China

⁴. CAS Key Laboratory of Genomic and Precision Medicine, Beijing Institute of Genomics, Chinese Academy of Sciences and China National Center for Bioinformation, Beijing 100101, China

⁵. University of Chinese Academy of Sciences, Beijing 100049, China

⁶. Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing 100101, China

⁷. Beijing Institute for Stem Cell and Regenerative Medicine, Beijing 100101, China

⁸. Advanced Innovation Center for Human Brain Protection and National Clinical Research Center for Geriatric Disorders, Xuanwu Hospital Capital Medical University, Beijing 100053, China

⁹. Aging Translational Medicine Center, International Center for Aging and Cancer, Beijing Municipal Geriatric Medical Research Center, Xuanwu Hospital, Capital Medical University, Beijing 100053, China

¹⁰. International Center for Aging and Cancer, Hainan Academy of Medical Sciences, Hainan Medical University, Haikou 571199, China

¹¹. Aging Biomarker Consortium, Beijing 100101, China

¹². Beijing Anzhen Hospital, Capital Medical University, Beijing Institute of Heart Lung and Blood Vessel Diseases, Beijing 100029, China

ghliu@ioz.ac.cn (G.-H. Liu)

zhangwq@big.ac.cn (W. Zhang)

sunshuhui327@163.com (S. Sun)

qujing@ioz.ac.cn (J. Qu)

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Ming-Heng Li, Xiaoyu Jiang, Yaobin Jing, Kaowen Yan, Shi-Jia Bi, Si Wang, Shuai Ma, Guang-Hui Liu, Weiqi Zhang, Shuhui Sun, Jing Qu. CRISPR-based screening pinpoints H2AZ1 as a driver of senescence in human mesenchymal stem cells. Protein Cell, 2025, 16(4): 293-299 DOI:10.1093/procel/pwae035

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