Ca<sup>2+</sup> binding to the C<sub>2</sub>E domain of otoferlin is required for hair cell exocytosis and hearing

Han Chen; Mehar Monga; Qinghua Fang; Loujin Slitin; Jakob Neef; Shashank S. Chepurwar; Regina Célia Mingroni Netto; Karina Lezirovitz; Alfredo Tabith,Jr.; Fritz Benseler; Nils Brose; Kathrin Kusch; Carolin Wichmann; Nicola Strenzke; Barbara Vona; Julia Preobraschenski; Tobias Moser

doi:10.1093/procel/pwad058

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Protein Cell ›› 2024, Vol. 15 ›› Issue (4) : 305-312. DOI: 10.1093/procel/pwad058

LETTER

Ca²⁺ binding to the C₂E domain of otoferlin is required for hair cell exocytosis and hearing

Han Chen¹^,²^,³^,⁴ ,
Mehar Monga²^,⁴^,⁵ ,
Qinghua Fang¹^,²^,³ ,
Loujin Slitin¹^,⁴^,⁶^,⁷ ,
Jakob Neef¹^,²^,³ ,
Shashank S. Chepurwar²^,⁴^,⁸ ,
Regina Célia Mingroni Netto⁹ ,
Karina Lezirovitz¹⁰ ,
Alfredo Tabith,Jr.¹¹ ,
Fritz Benseler¹² ,
Nils Brose²^,¹²^,¹³ ,
Kathrin Kusch¹^,¹⁴ ,
Carolin Wichmann²^,⁶^,⁷^,¹³ ,
Nicola Strenzke¹^,²^,⁸ ,
Barbara Vona¹^,¹⁵^,¹⁶ ,
Julia Preobraschenski²^,⁵^,¹³ ,
Tobias Moser¹^,²^,³^,¹³

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¹. Institute for Auditory Neuroscience and InnerEarLab, University Medical Center Göttingen, 37075 Göttingen, Germany

². Collaborative Research Center 889, University of Göttingen, 37075 Göttingen, Germany

³. Auditory Neuroscience and Synaptic Nanophysiology Group, Max Planck Institute for Multidisciplinary Sciences, 37075 Göttingen, Germany

⁴. Göttingen Graduate Center for Neurosciences, Biophysics and Molecular Biosciences, University of Göttingen, 37075 Göttingen, Germany

⁵. Biochemistry of Membrane Dynamics Group, Institute for Auditory Neuroscience, University Medical Center Göttingen, 37075 Göttingen, Germany

⁶. Molecular Architecture of Synapses Group, Institute for Auditory Neuroscience and InnerEarLab, University Medical Center Göttingen, 37075 Göttingen, Germany

⁷. Center for Biostructural Imaging of Neurodegeneration, University Medical Center Göttingen, 37075 Göttingen, Germany

⁸. Auditory Systems Physiology Group, Institute for Auditory Neuroscience and InnerEarLab, University Medical Center Göttingen, 37075 Göttingen, Germany

⁹. Departamento de Genética e Biologia Evolutiva, Centro de Pesquisas sobre o Genoma Humano e Células-Tronco, Instituto de Biociências, Universidade de São Paulo, São Paulo 05508-220, Brazil

¹⁰. Laboratório de Otorrinolaringologia/LIM32, Faculdade de Medicina, Hospital das Clínicas, Universidade de São Paulo, São Paulo, SP 05508-220, Brazil

¹¹. DERDIC, Pontifícia Universidade Católica de São Paulo, São Paulo 05508-220, Brazil

¹². Department of Molecular Neurobiology, Max Planck Institute for Multidisciplinary Sciences, 37075 Göttingen, Germany

¹³. Multiscale Bioimaging Cluster of Excellence (MBExC), University of Göttingen, 37075 Göttingen, Germany

¹⁴. Functional Auditory Genomics Group, Auditory Neuroscience and Optogenetics Laboratory, German Primate Center, 37077 Göttingen, Germany

¹⁵. Hearing Genomics Group, Institute for Auditory Neuroscience and InnerEarLab, University Medical Center Göttingen, 37075 Göttingen, Germany

¹⁶. Institute of Human Genetics, University Medical Center Göttingen, 37075 Göttingen, Germany

tmoser@gwdg.de

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Han Chen, Mehar Monga, Qinghua Fang, Loujin Slitin, Jakob Neef, Shashank S. Chepurwar, Regina Célia Mingroni Netto, Karina Lezirovitz, Alfredo Tabith,Jr., Fritz Benseler, Nils Brose, Kathrin Kusch, Carolin Wichmann, Nicola Strenzke, Barbara Vona, Julia Preobraschenski, Tobias Moser. Ca²⁺ binding to the C₂E domain of otoferlin is required for hair cell exocytosis and hearing. Protein Cell, 2024, 15(4): 305‒312 https://doi.org/10.1093/procel/pwad058

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