Understanding the phase separation characteristics of nucleocapsid protein provides a new therapeutic opportunity against SARS-CoV-2

Dan Zhao; Weifan Xu; Xiaofan Zhang; Xiaoting Wang; Yiyue Ge; Enming Yuan; Yuanpeng Xiong; Shenyang Wu; Shuya Li; Nian Wu; Tingzhong Tian; Xiaolong Feng; Hantao Shu; Peng Lang; Jingxin Li; Fengcai Zhu; Xiaokun Shen; Haitao Li; Pilong Li; Jianyang Zeng

doi:10.1007/s13238-021-00832-z

PDF(2244 KB)

Protein Cell ›› 2021, Vol. 12 ›› Issue (9) : 734-740. DOI: 10.1007/s13238-021-00832-z

LETTER

Understanding the phase separation characteristics of nucleocapsid protein provides a new therapeutic opportunity against SARS-CoV-2

Dan Zhao¹ ,
Weifan Xu²^,³ ,
Xiaofan Zhang¹ ,
Xiaoting Wang⁴ ,
Yiyue Ge⁵ ,
Enming Yuan¹ ,
Yuanpeng Xiong⁶ ,
Shenyang Wu⁷ ,
Shuya Li¹ ,
Nian Wu¹ ,
Tingzhong Tian¹ ,
Xiaolong Feng⁸ ,
Hantao Shu¹ ,
Peng Lang¹ ,
Jingxin Li⁵ ,
Fengcai Zhu⁵^,⁹ ,
Xiaokun Shen¹⁰ ,
Haitao Li³^,¹¹ ,
Pilong Li²^,³ ,
Jianyang Zeng¹

Author information +

History +

Cite this article

EndNote

Ris (Procite)

Bibtex

Download citation ▾

Dan Zhao, Weifan Xu, Xiaofan Zhang, Xiaoting Wang, Yiyue Ge, Enming Yuan, Yuanpeng Xiong, Shenyang Wu, Shuya Li, Nian Wu, Tingzhong Tian, Xiaolong Feng, Hantao Shu, Peng Lang, Jingxin Li, Fengcai Zhu, Xiaokun Shen, Haitao Li, Pilong Li, Jianyang Zeng. Understanding the phase separation characteristics of nucleocapsid protein provides a new therapeutic opportunity against SARS-CoV-2. Protein Cell, 2021, 12(9): 734‒740 https://doi.org/10.1007/s13238-021-00832-z

References

Publishing order | Descend order by publishing year | Descend order by cited within

[1]	Carlson, C.R., Asfaha, J.B., Ghent, C.M., Howard, C.J., Hartooni, N., and Morgan, D.O.(2020). Phosphorylation modulates liquidliquid phase separation of the SARS-CoV-2 N protein. bioRxiv. CrossRef Google scholar

[2]	Chang CK, Hou MH, Chang CF, Hsiao CD, Huang TH (2014) The SARS coronavirus nucleocapsid protein–forms and functions. Antiviral Res 103:39–50 CrossRef Google scholar

[3]	Cong, Y.Y., Ulasli, M., Schepers, H., Mauthe, M., V’kovski, P., Kriegenburg, F., Thiel, V., de Haan, C.A.M., and Reggiori, F. (2020). Nucleocapsid protein recruitment to replication-transcription complexes plays a crucial role in coronaviral life cycle. J Virol 94. CrossRef Google scholar

[4]	Ge YY, Tian TZ, Huang SL, Wan FP, Li JX, Li SY, Yang H, Hong LX, Wu N, Yuan EM (2020) A data-driven drug repositioning framework discovered a potential therapeutic agent targeting COVID-19. bioRxiv. CrossRef Google scholar

[5]	Hurst KR, Koetzner CA, Masters PS (2013) Characterization of a critical interaction between the coronavirus nucleocapsid protein and nonstructural protein 3 of the viral replicase-transcriptase complex. J Virol 87:9159–9172 CrossRef Google scholar

[6]	Iserman C, Roden C, Boerneke M, Sealfon R, McLaughlin G, Jungreis I, Park C, Boppana A, Fritch E, Hou YJ (2020) Specific viral RNA drives the SARS CoV-2 nucleocapsid to phase separate. bioRxiv. CrossRef Google scholar

[7]	Klein IA, Boija A, Afeyan LK, Hawken SW, Fan M, Dall’Agnese A, Oksuz O, Henninger JE, Shrinivas K, Sabari BR (2020) Partitioning of cancer therapeutics in nuclear condensates. Science 368:1386–1392 CrossRef Google scholar

[8]	Lin SY, Liu CL, Chang YM, Zhao J, Perlman S, Hou MH (2014) Structural basis for the identification of the N-terminal domain of coronavirus nucleocapsid protein as an antiviral target. J Med Chem 57:2247–2257 CrossRef Google scholar

[9]	Malik YA (2020) Properties of Coronavirus and SARS-CoV-2. Malays J Pathol 42:3–11

[10]	Nelson GW, Stohlman SA,Tahara SM (2000) High affinity interaction between nucleocapsid protein and leader/intergenic sequence of mouse hepatitis virus RNA. J General Virol 81:181–188 CrossRef Google scholar

[11]	Perdikari, T.M., Murthy, A.C., Ryan, V.H., Watters, S., Naik, M.T., and Fawzi, N.L. (2020). SARS-CoV-2 nucleocapsid protein undergoes liquid-liquid phase separation stimulated by RNA and partitions into phases of human ribonucleoproteins. bioRxiv. CrossRef Google scholar

[12]	Savastano, A.,Opakua, A.L., Rankovic, M., Zweckstetterc, M. (2020). Nucleocapsid protein of SARS-CoV-2 phase separates into RNA-rich polymerase-containing condensates. bioRxiv. CrossRef Google scholar

[13]	Stohlman SA, Baric RS, Nelson GN, Soe LH, Welter LM, Deans RJ (1988) Specific interaction between coronavirus leader RNA and nucleocapsid protein. J Virol 62:4288–4295 CrossRef Google scholar

[14]	Surjit M, Lal SK (2008) The SARS-CoV nucleocapsid protein: a protein with multifarious activities. Infect Genet Evol 8:397–405 CrossRef Google scholar