Rapid construction of a whole-genome mutant library by combining haploid stem cells and inducible self-inactivating PiggyBac transposon

Junjie Mao; Kai Xu; Jiabao Han; Guihai Feng; Ying Zhang; Wei Li

doi:10.1007/s13238-020-00702-0

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Protein Cell ›› 2020, Vol. 11 ›› Issue (6) : 452-457. DOI: 10.1007/s13238-020-00702-0

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Rapid construction of a whole-genome mutant library by combining haploid stem cells and inducible self-inactivating PiggyBac transposon

Junjie Mao¹^,²^,³ ,
Kai Xu¹^,²^,³ ,
Jiabao Han¹^,²^,³ ,
Guihai Feng¹^,²^,³ ,
Ying Zhang¹^,²^,³ ,
Wei Li¹^,²^,³

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Junjie Mao, Kai Xu, Jiabao Han, Guihai Feng, Ying Zhang, Wei Li. Rapid construction of a whole-genome mutant library by combining haploid stem cells and inducible self-inactivating PiggyBac transposon. Protein Cell, 2020, 11(6): 452‒457 https://doi.org/10.1007/s13238-020-00702-0

References

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[1]	Chen Y, Yee D, Dains K, Chatterjee A, Cavalcoli J, Schneider E, Om J, Woychik RP, Magnuson T (2000) Genotype-based screen for ENU-induced mutations in mouse embryonic stem cells. Nat Genet 24:314–317 CrossRef Google scholar

[2]	Ding S, Wu X, Li G, Han M, Zhuang Y, Xu T (2005) Efficient transposition of the piggyBac (PB) transposon in mammalian cells and mice. Cell 122:473–483 CrossRef Google scholar

[3]	Dupuy AJ, Akagi K, Largaespada DA, Copeland NG, Jenkins NA (2005) Mammalian mutagenesis using a highly mobile somatic Sleeping Beauty transposon system. Nature 436:221–226 CrossRef Google scholar

[4]	He ZQ, Xia BL, Wang YK, Li J, Feng GH, Zhang LL, Li YH, Wan HF, Li TD, Xu K (2017) Generation of mouse haploid somatic cells by small molecules for genome-wide genetic screening. Cell reports 20:2227–2237 CrossRef Google scholar

[5]	Horn C, Hansen J, Schnutgen F, Seisenberger C, Floss T, Irgang M, De-Zolt S, Wurst W, von Melchner H, Noppinger PR (2007) Splinkerette PCR for more efficient characterization of gene trap events. Nat Genet 39:933–934 CrossRef Google scholar

[6]	Kong J, Wang F, Brenton JD, Adams DJ (2010) Slingshot: a PiggyBac based transposon system for tamoxifen-inducible ‘selfinactivating’ insertional mutagenesis. Nucleic acids Res 38:e173 CrossRef Google scholar

[7]	Leeb M, Dietmann S, Paramor M, Niwa H, Smith A (2014) Genetic exploration of the exit from self-renewal using haploid embryonic stem cells. Cell Stem Cell 14:385–393 CrossRef Google scholar

[8]	Li W, Shuai L, Wan H, Dong M, Wang M, Sang L, Feng C, Luo GZ, Li T, Li X (2012) Androgenetic haploid embryonic stem cells produce live transgenic mice. Nature 490:407–411 CrossRef Google scholar

[9]	Liang Y, Li S, Chen L (2015) The physiological role of drug transporters. Protein Cell 6:334–350 CrossRef Google scholar

[10]	Robinson JT, Thorvaldsdottir H, Winckler W, Guttman M, Lander ES, Getz G, Mesirov JP (2011) Integrative genomics viewer. Nat Biotechnol 29:24–26 CrossRef Google scholar

[11]	Schneeberger K (2014) Using next-generation sequencing to isolate mutant genes from forward genetic screens. Nat Rev Genet 15:662–676 CrossRef Google scholar

[12]	Wang W, Bradley A, Huang Y (2009) A piggyBac transposon-based genome-wide library of insertionally mutated Blm-deficient murine ES cells. Genome Res 19:667–673 CrossRef Google scholar

[13]	Wang T, Wei JJ, Sabatini DM, Lander ES (2014) Genetic screens in human cells using the CRISPR-Cas9 system. Science 343:80–84 CrossRef Google scholar

[14]	Wray J, Kalkan T, Gomez-Lopez S, Eckardt D, Cook A, Kemler R, Smith A (2011) Inhibition of glycogen synthase kinase-3 alleviates Tcf3 repression of the pluripotency network and increases embryonic stem cell resistance to differentiation. Nat Cell Biol 13:838–845 CrossRef Google scholar