CRISPR/Cas9 and TALE: beyond cut and paste

Abstract

Nuclease-based genome editing has proven to be a powerful and promising tool for disease modeling and gene therapy. Recent advances in CRISPR/Cas and TALE indicate that they could also be used as a targeted regulator of gene expression, as well as being utilized for illuminating specific chromosomal structures or genomic regions.

Keywords

TALE / CRISPR/Cas9 / genome editing / gene expression / transcription regulation

References

[1] Chen B (2013) Dynamic imaging of genomic loci in living human cells by an optimized CRISPR/Cas system. Cell155: 1479-1491

[2] Gaj T, Gersbach CA, Barbas CF 3rd (2013) ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering. Trends Biotechnol31: 397-405

[3] Gilbert LA (2013) CRISPR-mediated modular RNA-guided regulation of transcription in eukaryotes. Cell154: 442-451

[4] Hsu PD (2013) DNA targeting speciflcity of RNA-guided Cas9 nucleases. Nat Biotechnol31: 827-832

[5] Konermann S (2013) Optical control of mammalian endogenous transcription and epigenetic states. Nature500: 472-476

[6] Konermann S (2014) Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex. Nature512(7515): 441-444

[7] Li M, Suzuki K, Kim NY, Liu GH, Izpisua Belmonte JC (2014) A cut above the rest: targeted genome editing technologies in human pluripotent stem cells. J Biol Chem289: 4594-4599

[8] Liu GH (2014) Modelling Fanconi anemia pathogenesis and therapeutics using integration-free patient-derived iPSCs. Nat Commun5: 4330

[9] Maeder ML (2013) Targeted DNA demethylation and activation of endogenous genes using programmable TALE-TET1 fusion proteins. Nat Biotechnol31: 1137-1142

[10] Ma H, Reyes-Gutierrez P, Pederson T (2013) Visualization of repetitive DNA sequences in human chromosomes with transcription activator-like effectors. Proc Natl Acad Sci USA110: 21048-21053

[11] Mendenhall EM (2013) Locus-speciflc editing of histone modiflcations at endogenous enhancers. Nat Biotechnol31: 1133-1136

[12] Miyanari Y, Ziegler-Birling C, Torres-Padilla ME (2013) Live visualization of chromatin dynamics with fluorescent TALEs. Nat Struct Mol Biol20: 1321-1324

[13] Pan H, Zhang W, Liu GH (2011) Find and replace: editing human genome in pluripotent stem cells. Protein Cell2: 950-956

[14] Smith C (2014) Whole-genome sequencing analysis reveals high speciflcity of CRISPR/Cas9 and TALEN-based genome editing in human iPSCs. Cell Stem Cell15: 12-13

[15] Suzuki K (2014) Targeted gene correction minimally impacts whole-genome mutational load in human-disease-speciflc induced pluripotent stem cell clones. Cell Stem Cell15: 31-36

[16] Therizols P (2014) Chromatin decondensation is sufflcient to alter nuclear organization in embryonic stem cells. Science346: 1238-1242

[17] Veres A (2014) Low incidence of off-target mutations in individual CRISPR-Cas9 and TALEN targeted human stem cell clones detected by whole-genome sequencing. Cell Stem Cell15: 27-30

[18] Zalatan JG (2014) Engineering complex synthetic transcriptional programs with CRISPR RNA scaffolds. Cell160: 339-350

RIGHTS & PERMISSIONS

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.