Distance measurement between two flexible sites in proteins in high viscosity medium at physiological temperature using continuous wave EPR

Lu Yu , Wei Wang , Shenglong Ling , Yao He , Liang Xiao , Kaiqi Wu , Longhua Zhang , Changlin Tian

Protein Cell ›› 2014, Vol. 5 ›› Issue (5) : 334 -337.

PDF (288KB)
Protein Cell ›› 2014, Vol. 5 ›› Issue (5) : 334 -337. DOI: 10.1007/s13238-014-0040-5
LETTER
LETTER

Distance measurement between two flexible sites in proteins in high viscosity medium at physiological temperature using continuous wave EPR

Author information +
History +
PDF (288KB)

Cite this article

Download citation ▾
Lu Yu, Wei Wang, Shenglong Ling, Yao He, Liang Xiao, Kaiqi Wu, Longhua Zhang, Changlin Tian. Distance measurement between two flexible sites in proteins in high viscosity medium at physiological temperature using continuous wave EPR. Protein Cell, 2014, 5(5): 334-337 DOI:10.1007/s13238-014-0040-5

登录浏览全文

4963

注册一个新账户 忘记密码

References

[1]

Altenbach C, Oh KJ, Trabanino RJ, Hideg K, Hubbell WL (2001) Estimation of inter-residue distances in spin labeled proteins at physiological temperatures: experimental strategies and practical limitations. Biochemistry40: 15471-15482

[2]

Banham JE, Baker CM, Ceola S, Day IJ, Grant GH, Groenen EJ, Rodgers CT, Jeschke G, Timmel CR (2008) Distance measurements in the borderline region of applicability of CW-EPR and DEER: a model study on a homologous series of spin-labelled peptides. J Magn Reson191: 202-218

[3]

Essen LO, Siegert R, Lehmann WD, Oesterhelt D (1998) Lipid patches in membrane protein oligomers: crystal structure of the bacteriorhodopsin–lipid complex. Proc Natl Acad Sci USA95: 11673-11678

[4]

Hellmich UA, Glaubitz C (2009) NMR and EPR studies of membrane transporters. Biol Chem390: 815-834

[5]

Hustedt EJ, Beth AH (1999) Nitroxide spin–spin interactions: applications to protein structure and dynamics. Annu Rev Biophys Biomol Struct28: 129-153

[6]

Lakshmi KV, Brudvig GW (2001) Pulsed electron paramagnetic resonance methods for macromolecular structure determination. Curr Opin Struct Biol11: 523-531

[7]

Li J, Shi C, Gao Y, Wu K, Shi P, Lai C, Chen L, Wu F, Tian C (2012) Structural studies of Mycobacterium tuberculosis Rv0899 reveal a monomeric membrane-anchoring protein with two separate domains. J Mol Biol415: 382-392

[8]

McHaourab HS, Lietzow MA, Hideg K, Hubbell WL (1996) Motion of spin-labeled side chains in T4 lysozyme. Correlation with protein structure and dynamics. Biochemistry35: 7692-7704

[9]

Prevo B, Peterman EJ (2014) Forster resonance energy transfer and kinesin motor proteins. Chem Soc Rev43: 1144-1155

[10]

Rabenstein MD, Shin YK (1995) Determination of the distance between two spin labels attached to a macromolecule. Proc Natl Acad Sci USA92: 8239-8243

[11]

Sale K, Song L, Liu YS, Perozo E, Fajer P (2005) Explicit treatment of spin labels in modeling of distance constraints from dipolar EPR and DEER. J Am Chem Soc127: 9334-9335

[12]

Sompornpisut P, Roux B, Perozo E (2008) Structural reflnement of membrane proteins by restrained molecular dynamics and solvent accessibility data. Biophys J95: 5349-5361

[13]

Wu K, Shi C, Li J, Wang H, Shi P, Chen L, Wu F, Xiong Y, Tian C (2013) Efflcient long-distance NMR-PRE and EPR-DEER restraints for two-domain protein structure determination. Protein Cell4: 893-896

RIGHTS & PERMISSIONS

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

AI Summary AI Mindmap
PDF (288KB)

571

Accesses

0

Citation

Detail

Sections
Recommended

AI思维导图

/