Bisindoylmaleimide I enhances osteogenic differentiation

Fangfang Zhou1,2, Huizhe Huang2(), Long Zhang1()

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PDF(662 KB)
Protein Cell ›› 2012, Vol. 3 ›› Issue (4) : 311-320. DOI: 10.1007/s13238-012-2027-4
RESEARCH ARTICLE

Bisindoylmaleimide I enhances osteogenic differentiation

  • Fangfang Zhou1,2, Huizhe Huang2(), Long Zhang1()
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Abstract

The Wnt/β-catenin and bone morphogenetic proteins (BMPs) pathways play important roles in controlling osteogenesis. Using a cell-based kinase inhibitor screening assay, we identified the compound bisindoylmaleimide I (BIM) as a potent agonist of the cytosolic β-catenin accumulation in preosteoblast cells. Through suppressing glycogen synthase kinase 3β enzyme activities, BIM upregulated β-catenin responsive transcription and extended duration of BMP initiated signal. Functional analysis revealed that BIM promoted osteoblast differentiation and bone formation. The treatment of human mesenchymal stem cells with BIM promoted osteoblastogenesis. Our findings provide a new strategy to regulate mesenchymal stem cell differentiation by integration of the cellular signaling pathways.

Keywords

bisindoylmaleimide I / Wnt/β-catenin / glycogen synthase kinase 3β / bone morphogenetic protein / human mesenchymal stem cells (hMSCs) / osteogenesis

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Fangfang Zhou, Huizhe Huang, Long Zhang. Bisindoylmaleimide I enhances osteogenic differentiation. Prot Cell, 2012, 3(4): 311‒320 https://doi.org/10.1007/s13238-012-2027-4

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