Magic Permutation: QBEmax Achieves High-Purity C-to-T Base Editing By Combining Cas9 Circular Permutation With Deaminase Domain Inlaying

References

[1] J. Hu, M. Guo, Q. Gao, et al., “QBEmax Is a Sequence-Permuted and Internally Protected Base Editor,” Nature Biotechnology, April 21, 2025, https://doi.org/10.1038/s41587-025-02641-9.

[2] A. C. Komor, Y. B. Kim, M. S. Packer, J. A. Zuris, and D. R. Liu, “Programmable Editing of a Target Base in Genomic DNA Without Double-Stranded DNA Cleavage,” Nature 533, no. 7603 (2016): 420–424.

[3] Y. Wang, L. Zhou, N. Liu, and S. Yao, “BE-PIGS: A Base-Editing Tool With Deaminases Inlaid Into Cas9 PI Domain Significantly Expanded the Editing Scope,” Signal Transduction and Targeted Therapy 4 (2019): 36.

[4] T. P. Huang, K. T. Zhao, S. M. Miller, et al., “Circularly Permuted and PAM-Modified Cas9 Variants Broaden the Targeting Scope of Base Editors,” Nature Biotechnology 37, no. 6 (2019): 626–631.

[5] L. Ye, D. Zhao, J. Li, et al., “Glycosylase-Based Base Editors for Efficient T-to-G and C-to-G Editing in Mammalian Cells,” Nature Biotechnology 42, no. 10 (2024): 1538–1547.

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2025 The Author(s). MedComm – Biomaterials and Applications published by John Wiley & Sons Australia, Ltd on behalf of Sichuan International Medical Exchange & Promotion Association (SCIMEA).