Effect of VEGF/GREDVY Modified Surface on Vascular Cells Behavior

Abstract

We synthesized B-He/B-GREDVY and immobilized them on avidin-coated surfaces. To examine the immobilization of molecules in the material, the following experiments were performed: fluorescein isothiocyanate (FITC) fluorescence staining, water contact angle and atomic force microscopy (AFM) measurements. Besides, the biological evaluation experiments were also performed, such as platelets adhesion and activation, the culturing of smooth muscle cells (SMC) and endothelial cells (EC). These experimental results show that the modified surfaces could prevent the hyperproliferation of SMC, and promote the proliferation and migration of EC and EPC. Furthermore, the adding of VEGF improved the EC adhesion in a dynamic environment. Generally, it is expected that the modified surfaces could be used to accelerate the formation of the newly endothelial layer for the construction of platforms for coronary artery stent therapy.

Keywords

biotin-GREDVY / VEGF / anticoagulation / endothelial cells / endothelial progenitor cells

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