Change in shape and crystal structure of HAP nanoparticles during absorption into cell

Abstract

The change of hydroxyapatite (HAP) nanoparticles in shape and crystal structure after endocytosis into cancer cells was studied. BEL7402 cells were incubated with HAP nanoparticles for 2 hour, 8 hours, 20 hours, respectively. Then, the cells were collected and viewed under a transmission electronic microscope (TEM). Electronic diffraction (ED) attached to TEM was used to detect the properties of the particles. The results show that HAP particles in the cytoplasm can be degraded in cytoplasm. The degradation process is prolonged by more than 20 hours. Thus, it is concluded that HAP nanoparticles would be degraded after kill cells or delivery gene.

Keywords

HAP nanoparticle / cancer cell / degradation

References

[1] Aoki H., Akao M., Kano S., . Effects of Hydroxyapatite-sol Cell Growth[J]. Report of the Institute for Medical & Dental Engineering, 1992, 26: 15-21.

[2] Sun E.J., Li S.P., Yan Y.H. Selective Inhibition of Hepatocarcinoma Cells by Apatite NPs[J]. Chinese Journal of Biomedical Engineering, 2002, 11(2): 67-71.

[3] Hu S., Yan Y., Wang Y., . Titanium Dioxide Nanoparticle Absorbed by Hepatoma Cells in Vitro[J]. Journal of Wuhan University of Technology-Materials Science Edition, 2005, 20(3): 31-34.

[4] Hu S., Yan Y., Wang Y., . Ultrastructure Change of Hepatoma Cells in Ultrastructure after Inorganic Crystal Nanoparticles Uptaken[J]. J. of Wuhan University of Technology-Materials Science Edition, 2005, 20(suppl): 35-37.

[5] Hu S., Li S., Yan Y., . Apoptosis of Cancer Cells Induced by HAP Nanoparticles[J]. Journal of Wuhan University of Technology-Materials Science, 2005, 20(4): 13-15.

[6] Couzin J. NPs Cut Tumors’ Supply Lines[J]. Science, 2002, 296: 2 314-2 315.

[7] Hood J. D., Bednarski M., Frausto R. Tumor Regression by Targeted Gene Delivery to the Neovasculature[J]. Science, 2002, 296: 2 404-2 407.

[8] Hans M.L., Lowman A.M. Biodegradable NPs for Drug Delivery and Targeting. Current Opinion in Solid State & Materials[J]. Science, 2002, 6(4): 319-327.

[9] Hu S., Yan Y., Wang Y., . The Study of HAP Nanoparticles Uptaken by Cells in Vitro[J]. Chinese Journal of Biomedical Engineering, 2005, 24(4): 482-485.

[10] Han Y., Li S., Wang X., . Synthesis and Sintering of Nanocrystalline Hydroxyapatite Powders by Citric Acid Sol-gel Combustion Method[J]. Materials Research Bulletin, 2004, 39(3): 25-32.

[11] Zhang H., Li S., Yan Y. Dissolution Behavior of Hydroxyapatite Powder in Hydrothermal Solution[J]. Ceramics International, 2001, 27(4): 451-454.