Simultaneous production of hydrogen and volatile fatty acids from anaerobic digestion of Macrocystis pyrifera biomass residues

Abstract

The Macrocystis pyrifera biomass residues (MPBRs) after extraction of algin could be applied in anaerobic fermentation. The effects of different pretreatment conditions, substrate concentrations and initial pH values on hydrogen and volatile fatty acid (VFA) production during the anaerobic fermentation of MPBRs were evaluated. The optimal pretreatment conditions, substrate concentration, initial pH values were determined as thermo-alkaline pretreatment at 100 °C with 0.1 mol/L NaOH, 40 g/L and 7.0, respectively. Under these conditions, the maximum hydrogen production was 11.38 mL/g (volatile solids, VS), which was approximately 23 times higher than that of untreated MPBRs. Furthermore, the maximum total volatile fatty acid (TVFA) yield was found to be 0.055 g/g (VS) and the VFA mainly consisted of acetic and butyric acids. The results indicate that the yield of TVFA is positively correlated with hydrogen production, and the MPBRs could produce hydrogen and TVFA simultaneously. In addition, thermo-alkaline pretreatment is proven to be the best method for hydrogen and VFA production.

Keywords

Macrocystis pyrifera biomass residues / pretreatment / fermentation / hydrogen

References

[1] HornS JBioenergy from brown seaweeds[M], 2000, Norway, Department of Biotechnology Norwegian University of Science and Technology NTNU Trondheim

[2] HornS J, MoenE, OstgaardK. Direct determination of alginate content in brown algae by near infrared (NIR) spectroscopy [J]. J Appl Phycol, 1999, 11(1): 9-13

[3] LiuH-y, WangG-ce. Fermentative hydrogen production from macroalgae Laminaria japonica using anaerobic mixed bacteria [J]. Int J Hydrogen Energ, 2014, 39(17): 1-6

[4] ParkJ I, LeeJ, SimS J, LeeJ H. Production of hydrogen from marine macroalgae biomass using anaerobic sewage sludge microflora [J]. Biotechnol Bioproc E, 2009, 14: 307-315

[5] WangY, WangH, FengX-q, WangX-f, HuangJ-xin. Biohydrogen production from cornstalk wastes by anaerobic fermentation with activated sludge [J]. Int J Hydrogen Energ, 2010, 35(7): 3092-3099

[6] GurungA, van GinkelS W, KangW C, QambraniN A, OhS E. Evaluation of marine biomass as a source of methane in batch tests: A lab-scale study [J]. Energy, 2010, 43(1): 396-401

[7] MarquezG P, ReichardtW T, AzanzaR V, KlockeM, MontanoM N. Thalassic biogas production from sea wrack biomass using different microbial seeds: Cow manure, marine sediment and sea wrack-associated microflora [J]. Bioresource Technol, 2013, 133: 612-617

[8] ParkJ H, YoonJ J, ParkH D, KimY J, LimD J, KimS H. Feasibility of biohydrogen production from Gelidium amansii [J]. Int J Hydrogen Energ, 2011, 36: 13997-14003

[9] SunJ-x, YuanX-z, ShiX-s, ChuC-f, GuoR-b, KongH-nan. Fermentation of Chlorella sp. for anaerobic bio-hydrogen production: Influences of inoculum-substrate ratio, volatile fatty acids and NADH [J]. Bioresource Technol, 2011, 102: 10480-10485

[10] HawkesF R, ForseyH, PremierG C, DinsdaleR M, HawkesD L, GuwyA J, MaddyJ, CherrymanS, ShineJ, AutyD. Fermentative production of hydrogen from a wheat flour industry co-product [J]. Bioresource Technol, 2008, 99: 5020-5029

[11] KapdanI K, KargiF. Bio-hydrogen production from waste materials [J]. Enzyme Microb Tech, 2006, 38(5): 569-582

[12] HanS K, ShinH S. Biohydrogen production by anaerobic fermentation of food waste [J]. Int J Hydrogen Energ, 2004, 29(6): 569-577

[13] LayJ J, FanK S, ChangJ, KuC H. Influence of chemical nature of organic wastes on their conversion to hydrogen by heat-shock digested sludge [J]. Int J Hydrogen Energ, 2003, 28(12): 1361-1367

[14] HoltzappleM T, GrandaC B. Carboxylate platform: The MixAlco process part 1: Comparison of three biomass conversion platforms [J]. Appl Biochem Biotech, 2009, 156(1-3): 95-106

[15] PhamT N, UmY, YoonH H. Pretreatment of macroalgae for volatile fatty acid production [J]. Bioresource Technol, 2013, 146: 754-757

[16] YangZ-m, GuoR-b, XuX-h, FanX-l, LiX-ping. Thermo-alkaline pretreatment of lipid-extracted microalgal biomass residues enhances hydrogen production [J]. J Chem Technol Biot, 2010, 86(3): 454-460

[17] YangZ-m, GuoR-b, XuX-h, FanX-l, LuoS-jun. Fermentative hydrogen production from lipid-extracted microalgal biomass residues [J]. Appl Energ, 2011, 88: 3468-3472

[18] YangZ-m, GuoR-b, XuX-h, FanX-l, LuoS-jun. Enhanced hydrogen production from lipid-extracted microalgal biomass residues through pretreatment [J]. Int J Hydrogen Energ, 2010, 35: 9618-9623

[19] APHA.Standard methods for the examination of waste and wastewater [M], 2000, Washington, DC, American Public Health Association

[20] BaccayR A, HashimotoA G. Acidogenic and methanogenic fermentation of causticized straw [J]. Biotechnol Bioeng, 1984, 26(8): 885-891

[21] PenaudV, DelgenesJ P, MolettaR. Thermo-chemical pretreatment of a microbial biomass: Influence of sodium hydroxide addition on solubilization and anaerobic biodegradability [J]. Enzyme Microb Tech, 1999, 25(3-5): 258-263

[22] XiaoN-d, ChenY-g, ChenA-h, FengL-yu. Enhanced bio-hydrogen production from protein wastewater by altering protein structure and amino acids acidification type [J]. Sci Rep-UK, 2014, 4: 3992

[23] LiY, HuaD-l, ZhangJ, GaoM-t, ZhaoY-x, XuH-p, LiangX-h, JinF-q, ZhangX-dong. Influence of inoculum to substrate ratios (ISRs) on the performance of anaerobic digestion of algal residues [J]. Ann Microbiol, 2013, 64(3): 955-960

[24] DechrugsaS, KantachoteD, ChaiprapatS. Effects of inoculum to substrate ratio, substrate mix ratio and inoculum source on batch co-digestion of grass and pig manure [J]. Bioresource Technol, 2013, 146101-108

[25] GinkelS V, SungS, LayJ J. Biohydrogen production as a function of pH and substrate concentration [J]. Environ Sci Technol, 2001, 35: 4726-4730

[26] van NielE W, ClaassenP A, StamsA J. Substrate and product inhibition of hydrogen production by the extreme thermophile, caldicellulosiruptor saccharolyticus [J]. Biotechnol Bioeng, 2003, 81(3): 255-262

[27] KimS H, HanS K, ShinH S. Effect of substrate concentration on hydrogen production and 16S rDNA-based analysis of the microbial community in a continuous fermenter [J]. Process Biochem, 2006, 41: 199-207

[28] PanC M, FanY T, HouH W. Fermentative production of hydrogen from wheat bran by mixed anaerobic cultures [J]. Ind Eng Chem Res, 2008, 47: 5812-5818

[29] PrakashamR S, BrahmaiahP, SathishT, Sambasiva RaoK R S. Fermentative biohydrogen production by mixed anaerobic consortia:Impact of glucose to xylose ratio [J]. Int J Hydrogen Energ, 2009, 34: 9354-9361

[30] AntonopoulouG, GavalaH N, SkiadasI V, LyberatosG. Effect of substrate concentration on fermentative hydrogen production from sweet sorghum extract [J]. Int J Hydrogen Energ, 2011, 36: 4843-4851

[31] GongM-l, RenN-q, TangJi. Start-up and continuous operation of bio-hydrogen production reactor at pH 5 [J]. Environ Sci, 2005, 26(2): 177-180

[32] LiuG-z, ShenJ-quan. Effects of culture and medium conditions on hydrogen production from starch using anaerobic bacteria [J]. J Biosci Bioeng, 2004, 98: 251-256

[33] KhanalS. Biological hydrogen production: Effects of pH and intermediate products[J]. Int J Hydrogen Energ, 2004

[34] CheongD, HansenC. Acidogenesis characteristics of natural, mixed anaerobes converting carbohydrate-rich synthetic wastewater to hydrogen [J]. Process Biochem, 2006, 41: 1736-1745

[35] HussyI, HawkesF R, DinsdaleR, HawkesD L. Continuous fermentative hydrogen production from a wheat starch co-product by mixed microflora [J]. Biotechnol Bioeng, 2003, 84(6): 619-626

[36] CohenA, DistelB, van DeursenA. Role of anaerobic spore-forming bacteria in the acidogenesis of glucose: Changes induced by discontinuous or low-rate feed supply [J]. Anton Leeuw Int J G, 1985, 51(2): 179-192

[37] ZengS-j, YuanX-z, ShiX-s, QiuY-ling. Effect of inoculum/substrate ratio on methane yield and orthophosphate release from anaerobic digestion of Microcystis spp [J]. J Hazard Mater, 2010, 178(1-3): 89-93

[38] LiJ-z, XuY-p, ZhangL-guo. Homoacetogens and hydrogen consumption in anaerobic activated sludge bio-hydrogen production system [J]. Sci Technol Rev, 2011, 24: 29-32

[39] ShiX-q, JungK W, KimD H, AhnY T, ShinH S. Direct fermentation of Laminaria japonica for biohydrogen production by anaerobic mixed cultures [J]. Int J Hydrogen Energ, 2011, 36: 5857-5864

[40] LevinD. Biohydrogen production: Prospects and limitations to practical application [J]. Int J Hydrogen Energ, 2004, 29(2): 173-185

[41] ChangF Y, LinC Y. Calcium effect on fermentative hydrogen production in an anaerobic up-flow sludge blanket system [J]. Water Sci Technol, 2006, 54(9): 105-112

[42] SunX-x, LiT, JiX-jing. Parameter effects and mechanism of fermentative bio-hydrogen production from cornstalks [J]. Mod Chem Ind, 2010, 30(6): 60-62