Comparative analysis of volatile constituents between herbal pair flos lonicerae-caulis lonicerae and its single herbs

Abstract

Gas chromatography-mass spectrometry (GC-MS) and the chemometric resolution method (alternative moving window factor analysis, AMWFA) were used for comparative analysis of volatile constituents in herbal pair (HP) flos lonicerae-caulis lonicerae (FL-CL) and its single herbs. The temperature-programmed retention index (PTRI) was also employed for the identification of compounds. In total, 44, 39, and 50 volatile chemical components in volatile oil of FL, CL and HP FL-CL were separately determined qualitatively and quantitatively, accounting for 87.22%, 94.54% and 90.08% total contents of volatile oil of FL, CL and HP FL-CL, respectively. The results show that there are 32 common volatile constituents between HP FL-CL and single herb FL, 33 common volatile constituents between HP FL-CL and single herb CL, and 10 new constituents in the volatile oil of HP FL-CL.

Keywords

herbal pair / flos lonicerae-caulis lonicerae / volatile oil / gas chromatography-mass spectrometry / alternative moving window factor analysis

References

[1] XuQ.-h., LiuL.-y., ZhaoR.-hua.Collection of drug pairs in traditional Chinese medicine [M], 1996, Beijing, China Press of Traditional Chinese Medicine: 142

[2] SuQ.-ying.Commonly used herbal pairs and compatibility in traditional Chinese medicine clinic [M], 1984, Beijing, Publishing House of People’s Heathy: 41

[3] ChenY.-mei.Traditional Chinese medicine chemistry [M], 1988, Jinan, Shandong Publishing House of Science and Technology: 14

[4] ZengZ. D., LiangY. Z., WangY. L., LiX. R., LiangL. M., XuQ. S., ZhaoC. X., LiB. Y., ChauF. T.. Alternative moving window factor analysis for comparison analysis between complex chromatographic data [J]. J Chromatogr A, 2006, 1107: 273-285

[5] ZengZ. D., LiangY. Z., JiangZ. H., ChauF. T., WangJ. R.. Quantification of target components in complex mixtures using alternative moving window factor analysis and two-step iterative constraint method [J]. Talanta, 2008, 74: 1568-1578

[6] CavaleiroC., SalgueiroL. R., MiguelM. G., ProencaD. A., CunhaA.. Analysis by gas chromatography-mass spectrometry of the volatile components of Teucrium lusitanicum and Teucrium algarbiensis [J]. J Chromatogr A, 2004, 1033: 187-190

[7] ZhaoC.-x., LiangY.-z., FangH.-z., LiX.-ning.. Temperature-programmed retention indices for gas chromatography-mass spectroscopy analysis of plant essential oils [J]. J Chromatogr A, 2005, 1096: 76-85

[8] ZhaoC.-x., LiX.-n., LiangY.-z., FangH.-z., HuangL.-f., GuoF.-qiu.. Comparative analysis of chemical components of essential oils from different samples of Rhododendron with the help of chemometrics methods [J]. Chemom Intell Lab Syst, 2006, 82: 218-228

[9] ChoI. H., ChoiH. K., KimY. S.. Difference in the volatile composition of pine-mushrooms (Tricholoma matsutake Sing.) according to their grades [J]. J Agric Food Chem, 2006, 54: 4820-4825

[10] Pharmacopoeia Committee of the People’s Republic of ChinaPharmacopoeia of the People’s Republic of China: Part I [M], 2005, Beijing, Chemical Industry Press

[11] VandendoolH., KratzP. D.. A generalization of the retention index system including linear temperature programmed gas-liquid partition chromatography [J]. J Chromatogr, 1963, 11: 463-471

[12] HuY., LiangY.-z., LiB.-y., LiX.-n., DuY.-ping.. Multicomponent spectral correlative chromatography applied to complex herbal medicines [J]. J Agric Food Chem, 2004, 52: 7771-7776

[13] ManneR., ShenH. L., LiangY. Z.. Subwindow factor analysis [J]. Chemom Intell Lab Syst, 1999, 45: 171-176

[14] LiX.-r., LiangY.-z., ZhouT., ZhangL.-x., HuC.-di.. Comparative analysis of volatile constituents between recipe jingfangsan and its single herbs by GC-MS combined with alternative moving window factor analysis method [J]. J Sep Sci, 2009, 32: 258-266

[15] ZengY.-x., ZhaoC.-x., LiangY.-z., YangH., FangH.-z., YiL.-z., ZengZ.-da.. Comparative analysis of volatile components from Clematis species growing in China [J]. Anal Chim Acta, 2007, 595: 328-339

[16] WangY.-m., YiL.-z., LiangY.-z., LiH.-d., YuanD.-l., GaoH.-y., ZengM.-mao.. Comparative analysis of essential oil components in Pericarpium Citri Reticulatae Viride and Pericarpium Citri Reticulatae by GC-MS combined with chemometric resolution method [J]. J Pharm Biomed Anal, 2008, 46: 66-74

[17] ChenY., LiX.-r., ZhaoJ., ZhouT., ZouQiao.. Chemical component analysis of volatile oil in drug pair Herba Ephedrae-Ramulus Cinnamomi by GC-MS and CRM [J]. J Cent South Univ Technol, 2007, 14(4): 509-513

[18] HuC.-d., LiX.-r., YuL.-f., XuG.-w., LiuS.-y., LiangY.-zeng.. Analysis of volatile components in herbal pair herba schizonepetae-ramulus cinnamomi [J]. J Cent South Univ Technol, 2008, 15(6): 791-795