Extraction optimization of coumarins from radix angelicae pubescentis by HPLC-DAD coupled with uniform design

Abstract

Uniform design was used to optimize extraction condition of direct refluence extraction of coumarins from the Chinese traditional medicine of radix angelicae pubescentis(Duhuo); the sum peak area of coumarins separated with high performance liquid chromatography (HPLC) at detection wavelength of 320 nm was considered as detection index, two factors of solvent concentration and extraction time were mainly studied at extraction temperature of 85°C and a volume ratio of solvent to sample of 10 : 1. Optimal subclass, quadric polynomial step by step aggression and neural network method were applied to process the experimental results. The results show that the first and second methods give the same factors combination (concentration of ethanol: 95%, extraction time: 3.6 h) and the second method is much better than the first one. The extraction model is consequently developed.

Keywords

radix angelicae pubescentis(Duhuo) / couamrins / uniform design / high performance liquid chromatagraphy-diode array detector(HPLC-DAD)

References

[1] LiuJ H, ZdchockeS, BauerR. A polyacetylenic and a coumarin from angelica pubescents f. biserrata[J]. Phytochemistry, 1998, 49(1): 211-213

[2] LiuJ H, ZschockeS, ReiningerE, et al.. Inhibitory effects of angelicae pubescents. f. biserrata on 5-lipoxygenase and cyclogenase [J]. Planta Medica, 1998, 78(1): 525-531

[3] OkuyamaT. Studies on the antitumor-promoting activity of naturally occurring substances. II: Inhibition of tumor-promoter-enhanced phospholipids metabolism by umbelliferous materials [J]. Chemical and Pharmaceutical Bulletin, 1990, 38(4): 1084-1089

[4] KoF N, LiouM J, HuangT F, et al.. Vasorelaxation of rat thoracic aorta caused by osthole isolated from angelica pubescents[J]. European Journal of Pharmacology, 1992, 219(1): 29-34

[5] LiRong-zhi, HeYun-qing, QiaoMing, et al.. Studies of the active constituents of the Chinese drug “du-hou” angelica pubescents[J]. Acta Pharmaccutica Sinica, 1989, 27(7): 546-551(in Chinese)

[6] LiuJiang-hua, XuSui-xu, YaoXin-sheng. Research progress of chemical constituents and pharmacology of angelica pubescents[J]. Journal of Shenyang College of Pharmacy, 1994, 11(3): 143-150(in Chinese)

[7] ZhangHan-qing, ChenWei-min, YuanChangqi. A comparative studies on coumarins constituents of Chinese crude drug “Chuanduhuo” and “Zheduhuo” Yuan et Shan[J]. Journal of Plant Research and Environment, 1993, 2(4): 60-62(in Chinese)

[8] ChenWei-min, YaoXin-mei, ZhangHan-qing. Determination of two coumarin contents in the root of angelica biserrata by HPLC method[J]. Journal of Plant Resources and Environment, 2000, 9(4): 51-52(in Chinese)

[9] WangTian-yong, YangWen-yuan. Simultaneous determination of scopletin and umbelliferone in angelica pubescents by RP-HPLC[J]. Fenxi Shiyanshi, 1996, 15(1): 17-20(in Chinese)

[10] ZhangCai-feng. Technics research of extraction by alcohol of angelica pubescents[J]. Modern Traditional Chinese Medicine, 2004, 5(5): 66-67(in Chinese)

[11] NieShi-ming, YangChang-juan. Optimizing the extraction process of angelica pubescents by orthogonal design[J]. Hubei Journal of Chinese Medicine, 2004, 26(9): 51-52(in Chinese)

[12] FangQ, YeungH W, LeungH W, et al.. Micellemediated extraction and preconcentration of ginsenosides from Chinese herbal medicine[J]. Journal of Chromatography A, 2000, 904(1): 47-55

[13] TangChun-hong, WangBo-chu, ChenQi, et al.. A new experimental design for screening Chinese medicine formula [J]. Colloids and Surfaces B: Biointerfaces, 2004, 36(2): 105-109 in Chinese)

[14] FangKai-taiUniform design and uniform design table [M], 1994, Beijing, Science Press(in Chinese)