White, translucent, glossy mucilaginous callus was initiated from the matur zygotic embryos explants on callus induction medium with 2,4-D, BA, and kinetin in the 3–9th week of culture. This type of callus induction occurred at a lower frequency with either α-naphthaleneacetic acid (NAA) or IBA (both 8 mg/L). White, translucent, glossy mucilaginous callus was embryogenic and mainly developed from the cotyledons of the mature zygotic embryo. Somatic embryos were formed on differentiation medium. Desiccation tolerance can be induced by culturing somatic embryos of loblolly pine (Pinus taeda L.) on medium supplemented with 50 μm abscisic acid (ABA) and/or 8.5% polyethylene glycol (PEG₆₀₀₀). Scanning electron microscopy of desiccated somatic embryos showed that the size and external morphology of the desiccation tolerant somatic embryos recovered to the pre-desiccation state within 24–36 h, whereas the sensitive somatic embryos did not recover and remained shriveled, after the desiccated somatic embryos had been rehydrated. Peroxidase activity of desiccated somatic embryos increased sharply after 3 days of desiccation treatment, and desiccation tolerant somatic embryos had higher peroxidase activity compared to sensitive somatic embryos. Higher peroxidase activity of desiccation tolerant somatic embryos was possibly advantage of catalyzing the reduction of H₂O₂ which was produced by drought stress, and protecting somatic embryos from oxidative damage.

The effect of different sources and levels of N on dry matter production, nutrient uptake and ionic balance ofLarlix gmelini was studied. The results showed that the growth of the plants fertilized with ammonium was not as good as the control treatment. The growth of the plants fertilized with ammonium nitrate did not differ significantly from that in control or nitrate treatment, but was better than that in the ammonium treatment. Total cation concentrations in shoots varied little with N level in the ammonium and ammonium nitrate treatments, while those in the shoot increased with N level in the nitrate treatment. The treatments had little effect on the anion concentrations in the shoot. In the roots, the concentrations of both cations and anions changed little except for SO₄ ²⁻ and Ca²⁺. There existed a higher carboxylate production in the plants fertilized with nitrate. The ratio between the production of carboxylate and the production of organic N Δ(C-A)/ΔN_org was constant with N supply in the plants receiving nitrate, but obviously declined with N supply for ammonium-fed plants. Δ (C-A)/ΔN_org values were intermediate between those of the nitrate and the ammonium-fed plants as for the mixed N source.

The isolation, culture and the active determination of poplar ice nucleation active (INA) bacteria and the inoculation tests in laboratory and field were conducted, and the varieties, distribution and number of poplar INA bacteria and its pathogenicity and freezing injury property were determined. The study results showed that the INA bacteria widely spread on poplar in Northeast China and caused the frozen injury for poplar under the frost condition in Spring or Autumn, which was the key factor to induce INA bacterial canker. Through evaluation and investigation of different poplar varieties and inoculation tests, fine disease-resistant varieties and strains of poplar suitable for Northeast China were selected. Further tests for strong seedling showed that burying cuttings in sand and covering with plastic film could effectively avoid the frostbite, frozen and drought damage, reduce INA bacteria infection, and promote poplar growth. INA bacterial canker was detected early by highly specialized antiserums of INA bacteria and the agglutinated test of ring-shaped boundary surface. The inducers such as streptomycin, phenylmercuric acetae, salicylic acid and heat-killed bacteria to immerse cuttings, have obvious induced disease-resistant effect. Before poplar sprouted in early spring, through spraying the solution of frostbite agent, the control effect also was obvious.

Through the methods of correlation analysis and main factor analysis, the relationship between the poplar INA bacterial canker and circumstances was analyzed and 9 main factors for affecting the disease were selected. Based on the comprehensive analysis of main factors and induced factors, the standard for risk grades of this disease was promoted and northeast region of China was divided into 4 districts with different risk grades: seriously occurring district, commonly occurring district, occasionally occurring district, and un-occurring district. Nonlinear regression analysis for six model curves showed that the Richard growth model was suitable for describing the temporal dynamics of poplar INA bacterial canker. By stepwise variable selection method, the multi-variable linear regression forecasting equation was set up to predict the next year’s disease index, and the GM(1,1) model was also set up by grey method to submit middle or long period forecast.

Four vegetation types, namely coniferous and broadleaved mixed forest, secondary deciduous broadleaved forest, open shrubs and meadow, with dwarf bamboo (Indocalamus wilsoni) are compared on their floristic composition, life form, community structure, and survivorship of bamboos. Disturbance of conifer-logging two decades ago promoted the species diversity, but negatively influenced the survivorship of dwarf bamboo which is dominate on the forest floor. Open shrubs bear more species diversity and more geophytes in its life-form spectrum than its background vegetation subalpine meadow, however, dwarf bamboo is growing much better in the open shrubs than in the meadow. It seems dwarf bamboo probably favorites to occur in the vegetation at gentle slopes with a mediate canopy cover. Its mass flowering in the secondary deciduous forest suggests that a simultaneous flowering and following dieback maybe irrupt in the recently coming years over the subalpine Shennongjia in China, which will be not only alter the floristic composition and community structure of the old bamboo stands, but also influence the survivorship of this rare species.

Seed germination process has closely relation with material transformation and energy exchange within the seed. Study on its thermal effect is important for understanding the mechanism and the influencing factors of the seed germination. The thermogenetic curves of seed germination ofRobinia pseudoacacia was measured by a new-type conductive microcalorimeter made in Wuhan University. The relationship was analyzed between the germination thermogenetic regulation and seed germination physiology. The thermogentic curves were further analyzed by thermokinetic theory to obtain the dynamic parameters and the thermokinetic model on seed germination ofRobinia pseudoacacia. The relationship of the thermogenetic power (μ w) and the germination time(h) of the germination process of 20 grainsRobinia pseudocacia seeds at 25°C wasP=208.77/[0.1937+0.8063exp(−0.06563t)]

According to the cultivating practice ofLarix olgensis pulp plantation, IRR (Internal revenue rate) and NPV (Net present value) were taken as two economic indices to study the effect of cultivation measurements on economic benefit ofLarix olgensis pulp forest. The results showed that the economic benefit of this type of forest is closely related to rotation and site class. Higher economic benefit could be obtained when the rotation is shorter and site class is higher. The planting density also had an obvious influence on economic benefit. On the base of assuring survival rate and conserving rate, the less the fee used in soil preparation and young growth tending is, the higher the economic benefit is. The influence of determined six cultivation measures on economic benefit in sequence was the rotation—site class—density—management fee level—young growth tending intensity—soil preparation methods.

Using the multi-temporal Landsat data and survey data of national resources, the authors studied the dynamics of cultivated land and landcover changes of typical ecological regions in China. The results of investigation showed that the whole distribution of the cultivated land shifted to Northeast and Northwest China, and as a result, the ecological quality of cultivated land dropped down. The seacoast and cultivated land in the area of Yellow River Mouth expanded by an increasing rate of 0.73 km·a⁻¹, with a depositing rate of 2.1 km·a⁻¹. The desertification area of the dynamic of Horqin Sandy Land increased from 60.02% of the total land area in 1970s to 64.82% in 1980s but decreased to 54.90% in early 1990s. As to the change of North Tibet lakes, the water area of the Namu Lake decreased by 38.58 km² from year 1970 to 1988, with a decreasing rate of 2.14 km²·a⁻¹.

Picea mongolica is an endemic and endangered species in China. Ecosystem made ofPicea mongolica is a special sandy forest ecosystem in China. It is found at ecotone between forest and steppe, or agricultural district and pas toral area. Based on investigation, this paper discussed the formation and distribution ofPicea mongolica and studied its nature according to ecotone theory. It is clarified thatPicea mongolica belongs toPicea meyeri series. That is to say, it became a local race through long-term adaptation to the local climate, then formed allopatric semi-species, and finally turned into a taxonomical species.Picea mongolica forest is a super zonal climax community developing in ecotone between forest zone and steppe zone.

Growth model is an efficient way to study growing process of some factors of plants quantitatively. Height growth of Korean pine (Pinus koraiensis) was studied by using Hyperbola equation, Logistic equation, Richards equation with three parameters, and Richards equation with four parameters in this paper. The results showed that Richards equation with four parameters was the most suitable and could be turned into other theoretical equations when some parameters were given different value. The maximum height of trees could be given in advance when using Richards equation with four parameters, and it was even more corresponding to reality. In addition, a height growth model with real height of fixed age as a parameter was discussed in this paper. This kind of growth model could be used to calculate height growth of a given tree effectively.

In 2000, the authors collected a great quantity of relevant data by investigating more than 50 nature reserves. Based on the analysis of development and management situation of the protected areas in China, the main problems were put forward, such as, no unified management for income and managing activities, lack of special guideline and effective supervision, lack of income and investment, investing financial difference in different provinces. All these problems caused the lack of funds for construction, as well as the damage of resources and environment. Furthermore the conserving activities have to transmit to the profits in many protected areas. Combined with these problems, the primary solution programmers also were put forward.

Through 5 years of phenological observations onLarix principis-rupprechtii Mayr. in primary seed orchard and studies on population and individuals of clones, the annual periodic phenological laws were revealed and the annual phenological periodic table was drawn up. The correlation between various phenophases, the air temperature and active accumulated temperature were analyzed and expounded. The authors also analyzed the similarities and differences of phenophases among clonal individuals as well as the blooming properties of male and female flowers at the same time. This study could provide theoretical reference for working out the production plan of improved varieties and other management measures in seed orchard ofLarix principis-rupprechtii.

Behaviors of sika deer in spring were studied by scan sampling, ad libitum sampling, and all-occurrence recording methods during 1998. The results showed that behaviors of sika deer in spring can be classified by seven categories: grazing, ruminating, bedding, moving, standing, drinking, alert, agonistic and other behaviors. Various behavioral models were more regular. Grazing behavior was a kind of mainly behavioral model.